Abstract
(Matrix-assisted) laser desorption/ionization ((MA)LDI) mass spectrometry imaging (MSI) has been driven by remarkable technological developments in the last couple of years. Although molecular information of a wide range of molecules including peptides, lipids, metabolites, and xenobiotics can be mapped, (MA)LDI MSI only leads to the detection of the most abundant soluble molecules in the cells and, consequently, does not provide access to the least expressed species, which can be very informative in the scope of disease research. Within a short period of time, numerous protocols and concepts have been developed and introduced in order to increase MSI sensitivity, including in situ tissue chemistry and solvent-free matrix depositions. In this chapter, we will discuss some of the latest developments in the field of high-sensitivity MSI using solvent-free matrix depositions and will detail protocols of two methods with their capability of enriching molecular MSI signal as demonstrated within our laboratory.
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References
Anderson DM, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, Schey KL (2014) High resolution MALDI imaging mass spectrometry of retinal tissue lipids. J Am Soc Mass Spectrom 25(8):1394–1403. doi:10.1007/s13361-014-0883-2
Angel PM, Spraggins JM, Baldwin HS, Caprioli R (2012) Enhanced sensitivity for high spatial resolution lipid analysis by negative ion mode matrix assisted laser desorption ionization imaging mass spectrometry. Anal Chem 84(3):1557–1564. doi:10.1021/ac202383m
Berry KA, Hankin JA, Barkley RM, Spraggins JM, Caprioli RM, Murphy RC (2011) MALDI imaging of lipid biochemistry in tissues by mass spectrometry. Chem Rev 111(10):6491–6512. doi:10.1021/cr200280p
Burnum KE, Cornett DS, Puolitaival SM, Milne SB, Myers DS, Tranguch S, Brown HA, Dey SK, Caprioli RM (2009) Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation. J Lipid Res 50(11):2290–2298. doi:10.1194/jlr.M900100-JLR200
Cavatorta V, Sforza S, Mastrobuoni G, Pieraccini G, Francese S, Moneti G, Dossena A, Pastorello EA, Marchelli R (2009) Unambiguous characterization and tissue localization of Pru P 3 peach allergen by electrospray mass spectrometry and MALDI imaging. J Mass Spectrom 44(6):891–897. doi:10.1002/jms.1562
Cazares LH, Troyer D, Mendrinos S, Lance RA, Nyalwidhe JO, Beydoun HA, Clements MA, Drake RR, Semmes OJ (2009) Imaging mass spectrometry of a specific fragment of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 2 discriminates cancer from uninvolved prostate tissue. Clin Cancer Res 15(17):5541–5551. doi:10.1158/1078-0432.CCR-08-2892
Cazares LH, Troyer DA, Wang B, Drake RR, Semmes OJ (2011) MALDI tissue imaging: from biomarker discovery to clinical applications. Anal Bioanal Chem 401(1):17–27. doi:10.1007/s00216-011-5003-6
Chaurand P (2011) Imaging mass spectrometry: current performance and upcoming challenges. Curr Trends Mass Spectrom 30–37. http://www.chromatographyonline.com/imaging-mass-spectrometry-current-performance-and-upcoming-challenges
Chaurand P (2012) Imaging mass spectrometry of thin tissue sections: a decade of collective efforts. J Proteomics 75:4883–4892. doi:10.1016/j.jprot.2012.04.005
Chaurand P, Schwartz SA, Capriolo RM (2004) Profiling and imaging proteins in tissue sections by MS. Anal Chem 76(5):87A–93A
Chaurand P, Norris JL, Cornett DS, Mobley JA, Caprioli RM (2006) New developments in profiling and imaging of proteins from tissue sections by MALDI mass spectrometry. J Proteome Res 5(11):2889–2900. doi:10.1021/pr060346u
Chaurand P, Cornett DS, Angel PM, Caprioli RM (2011) From whole-body sections down to cellular level, multiscale imaging of phospholipids by MALDI mass spectrometry. Mol Cell Proteomics 10(2):O110.004259. doi:10.1074/mcp.O110.004259
Chen Y, Allegood J, Liu Y, Wang E, Cachon-Gonzalez B, Cox TM, Merrill AH Jr, Sullards MC (2008) Imaging MALDI mass spectrometry using an oscillating capillary nebulizer matrix coating system and its application to analysis of lipids in brain from a mouse model of Tay-Sachs/Sandhoff disease. Anal Chem 80(8):2780–2788. doi:10.1021/ac702350g
Chughtai K, Heeren RM (2010) Mass spectrometric imaging for biomedical tissue analysis. Chem Rev 110(5):3237–3277. doi:10.1021/cr100012c
Debois D, Ongena M, Cawoy H, De Pauw E (2013) MALDI-FTICR MS imaging as a powerful tool to identify Paenibacillus antibiotics involved in the inhibition of plant pathogens. J Am Soc Mass Spectrom 24(8):1202–1213. doi:10.1007/s13361-013-0620-2
Dufresne M, Thomas A, Breault-Turcot J, Masson JF, Chaurand P (2013) Silver assisted laser desorption ionization for high spatial resolution imaging mass spectrometry of olefins from thin tissue sections. Anal Chem 85(6):3318–3324. doi:10.1021/ac3037415
Grey AC, Chaurand P, Caprioli RM, Schey KL (2009) MALDI imaging mass spectrometry of integral membrane proteins from ocular lens and retinal tissue. J Proteome Res 8(7):3278–3283. doi:10.1021/pr800956y
Guo S, Wang Y, Zhou D, Li Z (2014) Significantly increased monounsaturated lipids relative to polyunsaturated lipids in six types of cancer microenvironment are observed by mass spectrometry imaging. Sci Rep 4:5959. doi:10.1038/srep05959
Hankin JA, Barkley RM, Murphy RC (2007) Sublimation as a method of matrix application for mass spectrometric imaging. J Am Soc Mass Spectrom 18(9):1646–1652. doi:10.1016/j.jasms.2007.06.010
Jun JH, Song Z, Liu Z, Nikolau BJ, Yeung ES, Lee YJ (2010) High-spatial and high-mass resolution imaging of surface metabolites of Arabidopsis thaliana by laser desorption-ionization mass spectrometry using colloidal silver. Anal Chem 82(8):3255–3265. doi:10.1021/ac902990p
Jungmann JH, Heeren RM (2012) Emerging technologies in mass spectrometry imaging. J Proteomics. doi:10.1016/j.jprot.2012.03.022
Lemaire R, Menguellet SA, Stauber J, Marchaudon V, Lucot JP, Collinet P, Farine MO, Vinatier D, Day R, Ducoroy P, Salzet M, Fournier I (2007) Specific MALDI imaging and profiling for biomarker hunting and validation: fragment of the 11S proteasome activator complex, Reg alpha fragment, is a new potential ovary cancer biomarker. J Proteome Res 6(11):4127–4134. doi:10.1021/pr0702722
Lusis AJ (2000) Atherosclerosis. Nature 407(6801):233–241. doi:10.1038/35025203
MacAleese L, Stauber J, Heeren RM (2009) Perspectives for imaging mass spectrometry in the proteomics landscape. Proteomics 9(4):819–834. doi:10.1002/pmic.200800363
Maxfield FR, Tabas I (2005) Role of cholesterol and lipid organization in disease. Nature 438(7068):612–621. doi:10.1038/nature04399
Minerva L, Clerens S, Baggerman G, Arckens L (2008) Direct profiling and identification of peptide expression differences in the pancreas of control and ob/ob mice by imaging mass spectrometry. Proteomics 8(18):3763–3774. doi:10.1002/pmic.200800237
Morita Y, Ikegami K, Goto-Inoue N, Hayasaka T, Zaima N, Tanaka H, Uehara T, Setoguchi T, Sakaguchi T, Igarashi H, Sugimura H, Setou M, Konno H (2010) Imaging mass spectrometry of gastric carcinoma in formalin-fixed paraffin-embedded tissue microarray. Cancer Sci 101(1):267–273. doi:10.1111/j.1349-7006.2009.01384.x
Murphy RC, Hankin JA, Barkley RM, Zemski Berry KA (2011) MALDI imaging of lipids after matrix sublimation/deposition. Biochim Biophys Acta 1811(11):970–975. doi:10.1016/j.bbalip.2011.04.012
Norris JL, Caprioli RM (2013) Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research. Chem Rev 113(4):2309–2342. doi:10.1021/cr3004295
Nygren H, Malmberg P (2004) Silver deposition on freeze-dried cells allows subcellular localization of cholesterol with imaging TOF-SIMS. J Microsc 215(Pt 2):156–161. doi:10.1111/j.0022-2720.2004.01374.x
Patterson NH, Thomas A, Chaurand P (2014) Monitoring time-dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry. J Mass Spectrom 49(7):622–627. doi:10.1002/jms.3382
Patti GJ, Shriver LP, Wassif CA, Woo HK, Uritboonthai W, Apon J, Manchester M, Porter FD, Siuzdak G (2010a) Nanostructure-initiator mass spectrometry (NIMS) imaging of brain cholesterol metabolites in Smith-Lemli-Opitz syndrome. Neuroscience 170(3):858–864. doi:10.1016/j.neuroscience.2010.07.038
Patti GJ, Woo H-K, Yanes O, Shriver L, Thomas D, Uritboonthai W, Apon JV, Steenwyk R, Manchester M, Siuzdak G (2010b) Detection of carbohydrates and steroids by cation-enhanced nanostructure-initiator mass spectrometry (NIMS) for biofluid analysis and tissue imaging. Anal Chem 82(1):121–128. doi:10.1021/ac9014353
Puolitaival SM, Burnum KE, Cornett DS, Caprioli RM (2008) Solvent-free matrix dry-coating for MALDI imaging of phospholipids. J Am Soc Mass Spectrom 19(6):882–886. doi:10.1016/j.jasms.2008.02.013
Reyzer ML, Caprioli RM (2007) MALDI-MS-based imaging of small molecules and proteins in tissues. Curr Opin Chem Biol 11(1):29–35. doi:10.1016/j.cbpa.2006.11.035
Reyzer ML, Caldwell RL, Dugger TC, Forbes JT, Ritter CA, Guix M, Arteaga CL, Caprioli RM (2004) Early changes in protein expression detected by mass spectrometry predict tumor response to molecular therapeutics. Cancer Res 64(24):9093–9100. doi:10.1158/0008-5472.CAN-04-2231
Römpp A, Spengler B (2013) Mass spectrometry imaging with high resolution in mass and space. Histochem Cell Biol. doi:10.1007/s00418-013-1097-6
Schriemer DC, Li L (1996) Detection of high molecular weight narrow polydisperse polymers up to 1.5 million Daltons by MALDI mass spectrometry. Anal Chem 68(17):2721–2725. doi:10.1021/ac960442m
Schwamborn K, Caprioli RM (2010) Molecular imaging by mass spectrometry—looking beyond classical histology. Nat Rev Cancer 10(9):639–646. doi:10.1038/nrc2917
Shanta SR, Zhou LH, Park YS, Kim YH, Kim Y, Kim KP (2011) Binary matrix for MALDI imaging mass spectrometry of phospholipids in both ion modes. Anal Chem 83(4):1252–1259. doi:10.1021/ac1029659
Stauber J (2012) Quantitation by MS imaging: needs and challenges in pharmaceuticals. Bioanalysis 4(17):2095–2098. doi:10.4155/bio.12.187
Stoeckli M, Chaurand P, Hallahan DE, Caprioli RM (2001) Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat Med 7(4):493–496. doi:10.1038/86573
Stoeckli M, Staab D, Schweitzer A (2007) Compound and metabolite distribution measured by MALDI mass spectrometric imaging in whole-body tissue sections. Int J Mass Spectr 260(2–3):195–202. doi:10.1016/j.ijms.2006.10.007
Sun N, Walch A (2013) Qualitative and quantitative mass spectrometry imaging of drugs and metabolites in tissue at therapeutic levels. Histochem Cell Biol 140(2):93–104. doi:10.1007/s00418-013-1127-4
Thomas A, Chaurand P (2014) Advances in tissue section preparation for MALDI imaging MS. Bioanalysis 6(7):967–982. doi:10.4155/bio.14.63
Thomas A, Charbonneau JL, Fournaise E, Chaurand P (2012) Sublimation of new matrix candidates for high spatial resolution imaging mass spectrometry of lipids: Enhanced information in both positive and negative polarities after 1,5-diaminonapthalene deposition. Anal Chem 84(4):2048–2054. doi:10.1021/ac2033547
Thomas A, Patterson NH, Marcinkiewicz MM, Lazaris A, Metrakos P, Chaurand P (2013) Histology-driven data mining of lipid signatures from multiple imaging mass spectrometry analyses: application to human colorectal cancer liver metastasis biopsies. Anal Chem 85(5):2860–2866. doi:10.1021/ac3034294
Wisztorski M, Croix D, Macagno E, Fournier I, Salzet M (2008) Molecular MALDI imaging: an emerging technology for neuroscience studies. Dev Neurobiol 68(6):845–858. doi:10.1002/dneu.20623
Woods AS, Jackson SN (2006) Brain tissue lipidomics: direct probing using matrix-assisted laser desorption/ionization mass spectrometry. AAPS J 8(2):E391–E395. doi:10.1208/aapsj080244
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Thomas, A., Patterson, N.H., Dufresne, M., Chaurand, P. (2016). (MA)LDI MS Imaging at High Specificity and Sensitivity. In: Cramer, R. (eds) Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry. Springer, Cham. https://doi.org/10.1007/978-3-319-04819-2_7
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DOI: https://doi.org/10.1007/978-3-319-04819-2_7
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