Abstract
Bioremediation is the use of organisms for the treatment of soil pollution. Root colonizing symbiotic microorganisms such as arbuscular mycorrhizal fungi (AMF) are mainly involved in phytoremediation, that uses plants for soil remediation. Phytoremediation comprises a set of technologies that use various plants as a containment, destruction or extraction technique (EPA 2000). These techniques have received considerable interest in recent years because of potential cost savings compared to conventional non biological techniques. Different strategies of phytoremediation can be applied depending on the kind of pollutants. In all cases, vegetation reduces infiltration of water and erosion. Heavy metals cannot be degraded and can only be extracted (phytoextraction) from the soil or immobilized in a non toxic form (phytostabilization). AMF can help alleviate metal toxicity to plants by reducing metal translocation from root to shoot (Leyval et al. 1997). Therefore they may contribute to plant establishment and survival in heavy metal polluted sites and could be used as a complement to immobilization strategies. Phytoextraction mainly uses plants accumulating high concentrations of heavy metals, which can be harvested, discarded and even extracted to recover metals. For this purpose plants with various capacities for metal accumulation are used, like members of the Brassicaceae which are generally considered non mycorrhizal, but also other accumulators producing higher biomass, which can be mycotrophic. Organic pollutants such as polycylic aromatic hydrocarbons (PAH) can be transformed or degraded through microbial activity, which is commonly enhanced in the root zone (rhizodegradation). It is not known whether this enhanced degradation in the rhizosphere is due to plant exudates including enzymes, surfactants, and other physical/chemical effects, and/or to increased microbial activity. Another possible mechanism for degradation of organic pollutants could be directly dependent on plant metabolism. However, this is not a quantitatively important route for PAH (Binet et al. 2000a), and will not be discussed here. AMF may be beneficial for PAH rhizodegradation because they affect root exudation and root associated microbial populations and because, in some ways, they act as an extension of the roots outside the rhizosphere. They may potentially also have a direct effect on PAH degradation. Finally, AMF can be used in bioassays of soil quality or soil toxicity, due to their sensitivity towards a range of soil pollutants. In this way, they could be used to show that adequate soil quality has been re-established after remediation.
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References
Barea J M, Azcón-Aguilar C, Azcón R (1997) Interactions between mycorrhizal fungi and rhizosphere microorganisms within the context of sustainable soil-plant systems. In A.C., Gange and V.K., Brown (ed.), Multitrophic interactions in terestrial systems, pp 65–77
Berreck M, Haselwandter K (2001) Effect of the arbuscular mycorrhizal symbiosis upon uptake of cesium and other cations by plants. Mycorrhiza 10:275–280
Binet P, Portal J M, Leyval C (2000a) Fate of polycyclic aromatic hydrocarbons (PAH) in the rhizosphere and mycorrhizosphere of ryegrass. Plant Soil 227:207–213
Binet P, Portal J M, Leyval C (2000b) Dissipation of 3–6-ring polycyclic aromatic hydrocarbons in the rhizosphere of ryegrass. Soil Biol Biochem 32:2011–2017
Birch, L D, Bachofen R (1990) Effects of microorganisms on the environmental mobility of radionucleides. In J.M. Bollang and G. Stozky (ed.), Soil Biochemistry, vol. 6. Marcel Dekker, New York, p.483–527
Bolan N S, Robson A D, Barrow N J (1987) Effects of phosphorus application and mycorrhizal inoculation on root characteristics of subterranean clover and ryegrass in relation to phosphorus uptake. Plant Soil 104:294–298
Brundrett M C, Ashwath N, Jasper D A (1996) Mycorrhizas in the Kakadu region of tropical Australia. I. Propaguels of mycorrhizal fungi and soil properties in natural habitats. Plant Soil 184:159–171
Criquet S, Joner E J, Léglize P, Leyval C (2000) Effects of anthracene and mycorrhiza on the activity of oxidoreductases in the roots and the rhizosphere of lucerne(Medicago sativa L.). Biotechnol Lett 22:1733–1737
Cunningham S D, Anderson T, Schwab A P and Hsu F C (1996) Phytoremediation of soils contaminated with organic pollutants. Adv Agron 56:55–114
Cutright T J and Lee S (1994) Microorganisms and metabolic pathways for remediation of PAH in contaminated soil. Fresenius Environ Bull 3:413–421
del Val C, Barea J M, Azcon-Aguilar C (1999) Diversity of arbuscular mycorrhizal fungus populations in heavy-metal-contaminated soils. Appl Environ Microbiol 65:718–723
Diaz G, Azcon-Aguilar C, Honrubia M (1996) Influence of arbuscular mycorrhizae on heavy metal (Zn and Pb) uptake and growth of Lygeum spartum and Anthyllis cytisoides. Plant Soil 180:241-249
Dighton J, Clint G M, Poskitt J M (1991) Uptake and accumulation of 137Cs by upland grassland soil fungi: a potential pool of Cs immobilization. Mycol Res 95:1052–1056
Ebbs S, Kochian L V (1998) Phytoextraction of zinc by oat (Avena sativa) barley (Hordeum vulgare) and indian mustard (Brassica juncea). Environ Sci Technol 32:802–806
Ebbs S, Kochian L, Lasat M, Pence N, Jiang T (2000) An integrated investigation of the phytoremediation of heavy metal and radionuclide contaminated soils: From the laboratory to the field. In: Wise D L, Trantolo D J, Cichon E J, Inyang H I, Stottmeister U (eds) Bioremediation of contaminated soils. Dekker, New York, pp 745–769
El-Kherbawy M, Angle J S, Heggo A, Chaney R L (1989) Soil pH, rhizobia,and vesicular-arbuscular mycorrhizae inoculation effects on growth and heavy metal uptake of alfalfa (Medicago sativa L.). Biol Fertil Soil 8:61–65
EPA (2000) Introduction to phytoremediation, Cincinatti, Ohio p 70.
Ernst W H O (2000) Evolution of metal hyperaccumulation and phytoremediation hype. New Phytol 146:357–358
George E, Römheld V, Marschner H (1994) Contribution of mycorrhizal fungi to micronutrient uptake by plants. In: Manthey J A, Crowley D E, Luster D G (eds) Biochemistry of metal micronutrients in the rhizosphere. CRC Press, Boca Raton pp 93–109
Germida J J, Siciliano S D, de Freitas J R, Seib A M (1998) Diversity of root-associated bacteria associated with field-grown canola (Brassica napus L.) and wheat (Triticum aestivum L.). FEMS Microb Ecol 26: 43–50
Gildon A, Tinker P B (1983) Interactions of vesicular arbuscular mycorrhizal infection and heavy metals in plants. The effects of heavy metals on the development of vesicular-arbuscular mycorrhizas. New Phytol 95: 247–261
Graham J H, Leonard R T, Menge J A (1981) Membrane-mediated decrease in root exudation responsible for phosphorus inhibition of vesicular-arbuscular mycorrhiza formation. Plant Physiol 68:549–552
Griffiths B S, Ritz K, Ebblewhite N, Dobson G (1999) Soil microbial community structure: Effects of substrate loading rates. Soil Biol Biochem 31:145–153
Haselwandter K, Berreck M (1994) Accumulation of radionuclides in fungi. In: Winkelmann G, Winge D R (eds) Metal ions in fungi. Dekker, New York pp 259–277
Haselwandter K, Leyval C, Sanders F E (1994) Impact of arbuscular mycorrhizal fungi on plant uptake of heavy metals and radionuclides from soil. In: Gianinazzi S, Schüepp H (eds) Impact of arbuscular mycorrhizas on sustainable agricultural and natural ecosystems. Birkhäuser, Basel pp 179–189
Hetrick BAD, Wilson G W T, Figge D A H (1994) The Influence of mycorrhizal symbiosis and fertilizer amendments on establishment of vegetation in heavy metal mine spoil. Environ Poll 86:171–179
Hildebrandt U, Kaldorf M, Bothe H (1999) The zinc violet and its colonization by arbuscular mycorrhizal fungi. J Plant Physiol 154:709–717
Huang J, Cunningham S (1996) Lead phytoextraction: species variation in lead uptake and translocation. New Phytol 134:75–84
Jacquot E, vanTuinen D, Gianinazzi S, Gianinazzi V (1999) Monitoring species of arbuscular mycorrhizal fungi in planta and in soil by nested PCR: application to the study of the impact of sewage sludge. Plant Soil 226:179–188
Jasper D A, Abott L K, Robson A D (1991) The effect of soil disturbance on vesicular arbuscular mycorrhizal fungi in soils from different vegetations types. New Phytol 118:471–476
Joner E J, Johansen A, de la Cruz MAT, Szolar OJM, Loibner A, Portal JM, Leyval C Rhizosphere effects on microbial community structure, and dissipation and toxicity of PAH in spiked soil. Environ Sci Technol, submitted
Joner E J, Briones R, Leyval C (2000) Metal binding capacity of arbuscular mycorrhizal fungi. Plant Soil 226:227–234
Joner E J, Leyval C (2001a) Arbuscular mycorrhizal influence on clover and ryegrass grown together in a soil spiked with polycyclic aromatic hydrocarbons. Mycorrhiza 10:155–159
Joner E J, Leyval C (2001b) Time-course of heavy metal uptake in maize and clover as affected by different mycorrhiza inoculation regimes. Biol Fertil Soil, in press
Joner E J, Leyval C (1997) Uptake of 109Cd by roots and hyphae of a Glomus mosseae/Trifolium subterraneum mycorrhiza from soil amended with high and low concentrations of cadmium. New Phytol 135:353–360
Kaldorf M, Kuhn A J, Schröder W H, Hildebrandt U and Bothe H (1999) Selective element deposits in maize colonized by a heavy metal tolerance conferring arbuscular mycorrhizal fungus. J Plant Physiol 154:718–728
Konoplev A V, Vitkorova N V, Virchenko E P, Popov V E, Bulgakov A A, Desmet G M (1993) Influence of agricultural countermeasures on the ratio of different chemical forms of radionuclides in soil and soil solution. Sci Total Environ 137:147–162
Koomen I, McGrath S P, Giller K (1990) Mycorrhizal infection of clover is delayed in soils contaminated with heavy metals from past sewage sludge applications. Soil Biol Biochem 22:871–873
Laheurte F, Leyval C and Berthelin J (1990) Root exudates of maize, pine and beech seedlings influenced by mycorrhizal and bacterial inoculation. Symbiosis 9:111–116
Leyval C, Binet P (1998) Effect of polyaromatic hydrocarbons (PAHs) on arbuscular mycorrhizal colonization of plants. J Environ Qual 27:402–407
Leyval C, Singh B R, Joner E J (1995) Occurrence and infectivity of arbuscular-mycorrhizal fungi in some Norwegian soils influenced by heavy metals and soil properties. Water Air Soil Poll 84:203-216
Leyval C, Turnau K, Haselwandter K (1997) Interactions between heavy metals and mycorrhizal fungi in polluted soils: physiological, ecological and applied aspects. Mycorrhiza 7:139–153
Leyval C, Weissenhorn I (1995) Root colonization of maize by a Cd-sensitive and a Cd-tolerant Glomus mosseae and cadmium uptake in sand culture. Plant Soil 175:233–238
Linderman R G (1988) Mycorrhizal interactions with the rhizosphere microflora: The mycorrhizosphere effect. Phytopathology 78:366–371
Linderman R G (1991) Mycorrhizal interactions in the rhizosphere. In: Keister D L, Cregan P B (eds) The rhizosphere and plant growth. Kluwer Academic Publishers, Dordrecht pp 343–348
Loth C. (1996) Abundance of arbuscular mycorrhizal fungi spores at different natives sites in dependence of sludge applications. Bodenkultur 47:89–96
McGonigle T P, Miller M H (1996) Development of fungi below ground in association with plants growing in disturbed and undisturbed soil. Soil Biol Biochem 28:263–269
McGraw A C, Gamble J F, Schenk N C (1979) Vesicular-arbuscular mycorrhizal uptake of cesium-134 in two tropical pasture grass species. Phytopathology 69:1038
Olsson P A, Bádth E, Jakobsen I and Söderström B (1996) Soil bacteria respond to presence of roots but not to mycelium of arbuscular mycorrhizal fungi. Soil Biol Biochem 28:463–470
Pawlowska T E, Chaney R L, Chin M, Charvat I (2000) Effects of metal phytoextraction practices on the indigenous community of arbuscular mycorrhizal fungi at a metal-contaminated landfill. Appl Environ Microbiol 66:2526–2530
Perry J J (1979) Microbial cooxidation involving hydrocarbons. Microbiol Rev 43:59–72
Ravnskov S, Nybroe 0 and Jakobsen I (1999) Influence of an arbuscular mycorrhizal fungus on Pseudomonas fluorescens DF57 in rhizosphere and hyphosphere soil. New Phytol. 142:113–122
Reddell P, Milnes A R (1992) Mycorrhizas and other specialised nutrient-acquisition strategies - Their occurrence in woodland plants from Kakadu and their role in rehabilitation of waste rock dumps at a local uranium mine. Aust J Bot 40:223–242
Rogers R D, Williams S E (1986) Vesicular-arbuscular mycorrhiza: Influence on plant uptake of cesium and cobalt. Soil Biol Biochem 18:371–376
Salt D E, Blaylock M, Kumar N P B A, Dushenkov V, Ensley B D, Chet I, Raskin I (1995) Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants. Biotechnol 13:468–474
Salzer P, Corbiere H and Boller T (1999) Hydrogen peroxide accumulation in Medicago truncatula roots colonized by the arbuscular mycorrhiza-forming fungus Glomus intraradices. Planta 208:319–325
Schreiner R P, Bethlenfalvay G J (1995) Mycorrhizal interactions in sustainable agriculture. Crit Rev Biotechnol 15:271–285
Schwab A P, Banks M K (1994) Biologically mediated dissipation of polyaromatic hydrocarbons in the root zone. In: Anderson T A, Coats J R (eds) Bioremediation through rhizosphere technology. ACS Symposium Series 563. American Chemical Society, Washington pp 132–141
Segal M G (1993) Agricultural countermeasures following the deposition of radioactivity after a nuclear accident. Sci Total Environ 137:31–48
Shetty K G, Hetrick B A D, Figge D A H, Schwab A P (1994) Effects of mycorrhiza and other soil microbes on revegetation of heavy metal contaminated mine spoil. Environ Poll 86:181–188
Smith S E, Read D J (1997) Mycorrhizal symbiosis. Academic Press, San Diego
Thompson J P (1994) Inoculation with vesicular-arbuscular mycorrhizal fungi from cropped soil overcomes long-fallow disorder of linseed (Linum usitatissimum L.) by improving P and Zn uptake. Soil Biol Biochem 26:1133–1143
Tonin C., Vandenkoornhuyse P, Joner E, Straczek J, Leyval C (2001) Diversity of AM fungi in the rhizosphere of Viola calaminaria and effect of these fungi on heavy metal uptake by clover. Mycorrhiza 10:161–168
Turnau K, Kottke I, Oberwinkler F (1993) Element localisation in mycorrhizal roots of Pteridium aquilinium (L.) Kuhn collected from experimental plots treated with cadmiun dust. New Phytol 123:465–472
van Tichelen K K, Colpaert J V, Van Assche J A (1996) Development of arbuscular mycorrhizas in a heavy metal contaminated soil amended with a metal inmobilizing substance. In: Azcón-Aguilar C, Barea J M (eds) Mycorrhizae in integrated systems. from genes to plant development. European Comission, Luxemburg pp 479–482.
Vandenkoornhuyse P (1998) Effet des métaux sur la diversité des champignons mycorhiziens à arbuscules dans les sols, Ph-D Thesis, Henri Poincaré University, Nancy I
Wan M T, Rahe J E, Watts R G (1998) A new technique for determining the sublethal toxicity of pesticides to the vesicular-arbuscular mycorrhizal fungus Glomus intraradices. Environ Toxicol Chem 17:1421–1428
Weissenhorn I, Leyval C, Berthelin J (1995) Bioavailability of heavy metals and abundance of arbuscular mycorrhica in a soil polluted by atmospheric deposition from a smelter. Biol Fertil Soil 19:22–28
Weissenhorn I, Leyval C (1996) Spore germination of arbuscular-mycorrhizal (AM) fungi in soils differing in heavy metal content and other physicochemical properties. Eur J Soil Biol 32:165–172
White P J, Broadley M R (2000) Mechanisms of cesium uptake by plants. New Phytol 147:241–256
WHO (1983) Part I: Chemical, environmental and experimental data. In: Evaluation of the carcinogenic risk of chemicals to humans - polycyclic aromatic hydrocarbons. Volume 32. International Agency for Research on Cancer, Lyon, France
Wilson S C and Jones K C (1993) Bioremediation of soils contaminated with polynuclear aromatic hydrocarbons (PAHs): A review. Environ Pollut 88:229–249
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Leyval, C., Joner, E.J., del Val, C., Haselwandter, K. (2002). Potential of arbuscular mycorrhizal fungi for bioremediation. In: Gianinazzi, S., Schüepp, H., Barea, J.M., Haselwandter, K. (eds) Mycorrhizal Technology in Agriculture. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8117-3_14
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