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Fluorescence Techniques

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Analytical Biotechnology

Part of the book series: Methods and Tools in Biosciences and Medicine ((MTBM))

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Abstract

Fluorescence is a spectrochemical analysis method in which the molecules of the analyte are excited by irradiation at a certain wavelength and the emitted radiation at a longer wavelength is measured. This analysis method is widely accepted and is a powerful technique that is used for a variety of medical, pharmaceutical, environmental and biotechnological applications. The fields are diverse, e.g., detection of oil in water, oil sample fingerprinting, bacterial viability tests, chlorophyll measurement, monitoring of algae, membrane structure analysis, proliferation assays, DNA/RNA quantification, fluorescent tracers, brightening agents, protein quantification, enzyme assays, protein conformation studies, vitamins, toxin analysis, fluorescent proteins (e.g., GFP, YFP, and RFP), antibiotic testing, and many more.

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Authors
  1. Christian Mayer
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  2. Thomas G. M. Schalkhammer
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Editors and Affiliations

  1. TUDELFT, Kluyver Lab. for Biotechnology, Julianalaan 67, BC Delft, 2628, The Netherlands

    Thomas G. M. Schalkhammer

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© 2002 Springer Basel AG

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Mayer, C., Schalkhammer, T.G.M. (2002). Fluorescence Techniques. In: Schalkhammer, T.G.M. (eds) Analytical Biotechnology. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8101-2_2

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  • DOI: https://doi.org/10.1007/978-3-0348-8101-2_2

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-7643-6589-9

  • Online ISBN: 978-3-0348-8101-2

  • eBook Packages: Springer Book Archive

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