Abstract
In research and development, cell disruption is vital to the acquisition of internal components, including metabolites and proteins. This is essential for producing many biological products, such as enzymes and antigens used to produce vaccines and nucleic acids. For cell lysis, various mechanical techniques exist, including high-pressure homogenization, sonication, bead milling, and many others. The growing importance of nanoparticles (NPs) in theranostics (therapeutic diagnostics), target modulation of cellular processes, etc., has been serving as a novel approach towards biotechnological applications such as silica-based nanoparticles (SNs) being used in drug delivery, imaging, and other therapeutic majors. The disruption of the cell caused by the biophysical interlinkage among the cell and nanoparticle allows researchers to use nanoparticles as drug carrier systems. This chapter discusses the biophysiochemical interactions between the cell and the nanomaterials, which serves as an essential factor for the disruption of cells, and also explains the methods used for the cell disruption along with current trends associated.
Anjali Raghav, Simran Kaur, Gunjit Setia—contributed equally to this work.
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References
Attama A (2011) SLN, NLC, LDC: state of the art in drug and active delivery. Recent PatS Drug Deliv Formul 5(3):178–187. https://doi.org/10.2174/187221111797200524
Andrews BA, Asenjo JA (1987) Enzymatic lysis and disruption of microbial cells. Trends Biotechnol 5(10):273–277. https://doi.org/10.1016/0167-7799(87)90058-8
Augenstein DC, Thrasher K, Sinskey AJ, Wang DIC (1974) Optimization in the recovery of a labile intracellular enzyme. Biotechnol Bioeng 16(11):1433–1447. https://doi.org/10.1002/BIT.260161102
Augustine R, Hasan A, Primavera R, Wilson RJ, Thakor AS, Kevadiya BD (2020) Cellular uptake and retention of nanoparticles: insights on particle properties and interaction with cellular components. Mater Today Commun 25:101692. https://doi.org/10.1016/J.MTCOMM.2020.101692
Baalousha M (2009) Aggregation and disaggregation of iron oxide nanoparticles: Influence of particle concentration, pH and natural organic matter. Sci Total Environ 407(6):2093–2101. https://doi.org/10.1016/J.SCITOTENV.2008.11.022
Banquy X, Suarez F, Argaw A, Rabanel JM, Grutter P, Bouchard JF, Hildgen P, Giasson S (2009) Effect of mechanical properties of hydrogel nanoparticles on macrophage cell uptake. Soft Matter 5(20):3984–3991. https://doi.org/10.1039/B821583A
Bapat RA, Chaubal TV, Joshi CP, Bapat PR, Choudhury H, Pandey M, Gorain B, Kesharwani P (2018) An overview of application of silver nanoparticles for biomaterials in dentistry. Mater Sci Engineering C, Mater Biol Appl 91:881–898. https://doi.org/10.1016/J.MSEC.2018.05.069
Bastos PAD, Wheeler R, Boneca IG (2021) Uptake, recognition and responses to peptidoglycan in the mammalian host. FEMS Microbiol Rev 45(1). https://doi.org/10.1093/FEMSRE/FUAA044
Belin BJ, Busset N, Giraud E, Molinaro A, Silipo A, Newman DK (2018) Hopanoid lipids: from membranes to plant-bacteria interactions. Nat Rev Microbiol 16(5):304–315. https://doi.org/10.1038/NRMICRO.2017.173
Beningo KA, Wang YL (2002) Fc-receptor-mediated phagocytosis is regulated by mechanical properties of the target. J Cell Sci 115(Pt 4):849–856. https://doi.org/10.1242/JCS.115.4.849
Bhardwaj V, Kaushik A (2017) Biomedical applications of nanotechnology and nanomaterials. Micromachines 8(10):298. https://doi.org/10.3390/MI8100298
Breunig M, Bauer S, Goepferich A (2008) Polymers and nanoparticles: intelligent tools for intracellular targeting? Eur J Pharm Biopharm 68(1):112–128. https://doi.org/10.1016/J.EJPB.2007.06.010
Brown RB, Audet J (2008) Current techniques for single-cell lysis. J R Soc Interface 5(Suppl 2). https://doi.org/10.1098/RSIF.2008.0009.FOCUS
Buyukhatipoglu K, Clyne AM (2011) Superparamagnetic iron oxide nanoparticles change endothelial cell morphology and mechanics via reactive oxygen species formation. J Biomed Mater Res, Part A 96(1):186–195. https://doi.org/10.1002/JBM.A.32972
Buzea C, Pacheco II, Robbie K (2007) Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2(4):MR17–MR71. https://doi.org/10.1116/1.2815690
Cameron SJ, Sheng J, Hosseinian F, Willmore WG (2022) Nanoparticle effects on stress response pathways and nanoparticle-protein interactions. Int J Mol Sci 23(14). https://doi.org/10.3390/IJMS23147962
Caviglia C (2015) Hydrodynamic cavitation as a method for cell disruption. https://digitalcommons.utep.edu/open_etd
Champion JA, Mitragotri S (2006) Role of target geometry in phagocytosis. Proc Natl Acad Sci USA 103(13):4930–4934. https://doi.org/10.1073/PNAS.0600997103
Chen J, Hessler JA, Putchakayala K, Panama BK, Khan DP, Hong S, Mullen DG, DiMaggio SC, Som A, Tew GN, Lopatin AN, Baker JR, Holl MMB, Orr BG (2009) Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes. J Phys Chem B 113(32):11179–11185. https://doi.org/10.1021/JP9033936
Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y, Qiu Y, Wang J, Liu Y, Wei Y, **a J, Yu T, Zhang X, Zhang L (2020) Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet (london, England) 395(10223):507–513. https://doi.org/10.1016/S0140-6736(20)30211-7
Chen T, Shukoor MI, Wang R, Zhao Z, Yuan Q, Bamrungsap S, **ong X, Tan W (2011) Smart multifunctional nanostructure for targeted cancer chemotherapy and magnetic resonance imaging. ACS Nano 5(10):7866. https://doi.org/10.1021/NN202073M
Chisti Y, Moo-Young M (1986) Disruption of microbial cells for intracellular products. Enzyme Microb Technol 8(4):194–204. https://doi.org/10.1016/0141-0229(86)90087-6
Del Prado-Audelo ML, García Kerdan I, Escutia-Guadarrama L, Reyna-González JM, Magaña JJ, Leyva-Gómez G (2021) Nanoremediation: nanomaterials and nanotechnologies for environmental cleanup. Front Environ Sci 9:645. https://doi.org/10.3389/FENVS.2021.793765/BIBTEX
Dörr T, Moynihan PJ, Mayer C (2019) Editorial: bacterial cell wall structure and dynamics. Front Microbiol 10:2051. https://doi.org/10.3389/FMICB.2019.02051/BIBTEX
Engler CR, Robinson CW (1981) Disruption of Candida utilis cells in high pressure flow devices*. Biotechnol Bioeng 23(4):765–780. https://doi.org/10.1002/BIT.260230408
Etheridge ML, Campbell SA, Erdman AG, Haynes CL, Wolf SM, McCullough J (2013) The big picture on nanomedicine: the state of investigational and approved nanomedicine products. Nanomed Nanotechnol Biol Med 9(1):1–14. https://doi.org/10.1016/J.NANO.2012.05.013
Feliciello I, Chinali G (1993) A modified alkaline lysis method for the preparation of highly purified plasmid DNA from escherichia coli. Anal Biochem 212(2):394–401. https://doi.org/10.1006/ABIO.1993.1346
Fercher A, Borisov SM, Zhdanov AV, Klimant I, Papkovsky DB (2011) Intracellular O2 sensing probe based on cell-penetrating phosphorescent nanoparticles. ACS Nano 5(7):5499–5508. https://doi.org/10.1021/NN200807G/SUPPL_FILE/NN200807G_SI_001.PDF
Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T (2003) In vitro cytotoxicity testing of polycations: Influence of polymer structure on cell viability and hemolysis. Biomaterials 24(7):1121–1131. https://doi.org/10.1016/S0142-9612(02)00445-3
Fonseca LP, Cabral JMS (2002) Penicillin acylase release from Escherichia coli cells by mechanical cell disruption and permeabilization. J Chem Technol Biotechnol 77(2):159–167. https://doi.org/10.1002/JCTB.541
Foroozandeh P, Aziz AA (2018) Insight into cellular uptake and intracellular trafficking of nanoparticles. Nanoscale Res Lett 13(1):1–12. https://doi.org/10.1186/S11671-018-2728-6
Gatoo MA, Naseem S, Arfat MY, Mahmood Dar A, Qasim K, Zubair S (2014) Physicochemical properties of nanomaterials: implication in associated toxic manifestations. BioMed Res Int. https://doi.org/10.1155/2014/498420
Ginzburg VV, Balijepalli S (2007) Modeling the thermodynamics of the interaction of nanoparticles with cell membranes. Nano Lett 7(12):3716–3722. https://doi.org/10.1021/NL072053L
Gobbi M, Re F, Canovi M, Beeg M, Gregori M, Sesana S, Sonnino S, Brogioli D, Musicanti C, Gasco P, Salmona M, Masserini ME (2010) Lipid-based nanoparticles with high binding affinity for amyloid-beta1-42 peptide. Biomaterials 31(25):6519–6529. https://doi.org/10.1016/J.BIOMATERIALS.2010.04.044
Goldberg S (2008) Mechanical/physical methods of cell disruption and tissue homogenization. Methods Mol Biol (Clifton, N.J.) 424:3–22. https://doi.org/10.1007/978-1-60327-064-9_1
Gomes TA, Zanette CM, Spier MR (2020) An overview of cell disruption methods for intracellular biomolecules recovery. Prep Biochem Biotechnol 50(7):635–654. https://doi.org/10.1080/10826068.2020.1728696
Guo M, Xu X, Yan X, Wang S, Gao S, Zhu S (2013) In vivo biodistribution and synergistic toxicity of silica nanoparticles and cadmium chloride in mice. J Hazard Mater 260:780–788. https://doi.org/10.1016/J.JHAZMAT.2013.06.040
Harrison STL (1991) Bacterial cell disruption: a key unit operation in the recovery of intracellular products. Biotechnol Adv 9(2):217–240. https://doi.org/10.1016/0734-9750(91)90005-G
Heng BC, Zhao X, Tan EC, Khamis N, Assodani A, **ong S, Ruedl C, Ng KW, Loo JSC (2011) Evaluation of the cytotoxic and inflammatory potential of differentially shaped zinc oxide nanoparticles. Arch Toxicol 85(12):1517–1528. https://doi.org/10.1007/S00204-011-0722-1
Hillaireau H, Couvreur P (2009) Nanocarriers’ entry into the cell: relevance to drug delivery. Cell Mol Life Sci: CMLS 66(17):2873–2896. https://doi.org/10.1007/S00018-009-0053-Z
Huh AJ, Kwon YJ (2011) “Nanoantibiotics”: a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. J Control Release: off J Control Release Soc 156(2):128–145. https://doi.org/10.1016/J.JCONREL.2011.07.002
Huq MA, Ashrafudoulla M, Rahman MM, Balusamy SR, Akter S (2022) green synthesis and potential antibacterial applications of bioactive silver nanoparticles: a review. Polymers 14(4):742. https://doi.org/10.3390/POLYM14040742
Iravani S (2020) Nanomaterials and nanotechnology for water treatment: recent advances. 51(12):1615–1645. https://doi.org/10.1080/24701556.2020.1852253
Islam MS, Aryasomayajula A, Selvaganapathy PR (2017) A review on macroscale and microscale cell lysis methods. Micromachines 8(3). https://doi.org/10.3390/MI8030083
Johnson BH, Hecht MH (1994) Recombinant proteins can be isolated from E. coli cells by repeated cycles of freezing and thawing. Bio/Technology (Nature Publishing Company) 12(13):1357–1360. https://doi.org/10.1038/NBT1294-1357
Kaushik AK, Dixit CK (2016) Nanobiotechnology for sensing applications : from lab to field
Khan YS, Farhana A (2022) Histology, cell. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK554382/
Khorrami S, Zarrabi A, Khaleghi M, Danaei M, Mozafari MR (2018) Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. Int J Nanomed 13:8013–8024. https://doi.org/10.2147/IJN.S189295
Kodali V, Thrall BD (2015) Oxidative stress and nanomaterial-cellular interactions. Stud Exp Toxicol Pharmacol 347–367. https://doi.org/10.1007/978-3-319-19096-9_18/COVER
Kumar V, Arora N, Nanda M, Pruthi V (2019) Different cell disruption and lipid extraction methods from microalgae for biodiesel production. Microalgae Biotechnol Dev Biofuel Wastewater Treat 265–292. https://doi.org/10.1007/978-981-13-2264-8_12
Laux P, Tentschert J, Riebeling C, Braeuning A, Creutzenberg O, Epp A, Fessard V, Haas KH, Haase A, Hund-Rinke K, Jakubowski N, Kearns P, Lampen A, Rauscher H, Schoonjans R, Störmer A, Thielmann A, Mühle U, Luch A (2018) Nanomaterials: certain aspects of application, risk assessment and risk communication. Arch Toxicol 92(1):121–141. https://doi.org/10.1007/S00204-017-2144-1
Lee NY, Ko WC, Hsueh PR (2019) Nanoparticles in the treatment of infections caused by multidrug-resistant organisms. Front Pharmacol 10:1153. https://doi.org/10.3389/FPHAR.2019.01153/BIBTEX
Leroueil PR, Berry SA, Duthie K, Han G, Rotello VM, McNerny DQ, Baker JR, Orr BG, Holl MMB (2008) Wide varieties of cationic nanoparticles induce defects in supported lipid bilayers. Nano Lett 8(2):420–424. https://doi.org/10.1021/NL0722929
Leroueil PR, Hong S, Mecke A, Baker JR, Orr BG, Holl MMB (2007) Nanoparticle interaction with biological membranes: does nanotechnology present a janus face? Acc Chem Res 40(5):335–342. https://doi.org/10.1021/AR600012Y
Liu JL, Zhang WJ, Li XD, Yang N, Pan WS, Kong J, Zhang JS (2016) Sustained-release genistein from nanostructured lipid carrier suppresses human lens epithelial cell growth. Int J Ophthalmol 9(5):643. https://doi.org/10.18240/IJO.2016.05.01
Lopez MJ, Hall CA (2022) Physiology, osmosis. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK557609/
Mahalanabis M, Al-Muayad H, Kulinski MD, Altman D, Klapperich CM (2009) Cell lysis and DNA extraction of gram-positive and gram-negative bacteria from whole blood in a disposable microfluidic chip. Lab Chip 9(19):2811–2817. https://doi.org/10.1039/B905065P
Majumder N (2015) Click chemistry in nano drug delivery system and its applications in biology. Int J Res Pharm Chem 5(1):95–105. www.ijrpc.com
Manke A, Wang L, Rojanasakul Y (2013) Mechanisms of nanoparticle-induced oxidative stress and toxicity. BioMed Res Int. https://doi.org/10.1155/2013/942916
Mecke A, Majoros IJ, Patri AK, Baker JR, Banaszak Holl MM, Orr BG (2005) Lipid bilayer disruption by polycationic polymers: the roles of size and chemical functional group. Langmuir: ACS J Surf Colloids 21(23):10348–10354. https://doi.org/10.1021/LA050629L
Middelberg APJ (2000) 2 microbial cell disruption by high-pressure homogenization, pp 11–21. https://doi.org/10.1007/978-1-59259-027-8_2
Moschini E, Colombo G, Chirico G, Capitani G, Dalle-Donne I, Mantecca P (2023) Biological mechanism of cell oxidative stress and death during short-term exposure to nano CuO. Sci Rep 13(1):1–18. https://doi.org/10.1038/s41598-023-28958-6
Mühling M, Bradford A, Readman JW, Somerfield PJ, Handy RD (2009) An investigation into the effects of silver nanoparticles on antibiotic resistance of naturally occurring bacteria in an estuarine sediment. Mar Environ Res 68(5):278–283. https://doi.org/10.1016/J.MARENVRES.2009.07.001
Ng KK, Lovell JF, Zheng G (2011) Lipoprotein-inspired nanoparticles for cancer theranostics. Acc Chem Res 44(10):1105. https://doi.org/10.1021/AR200017E
Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? a study of the Gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73(6):1712–1720. https://doi.org/10.1128/AEM.02218-06
Panariti A, Miserocchi G, Rivolta I (2012) The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions? Nanotechnol Sci Appl 5(1):87. https://doi.org/10.2147/NSA.S25515
Patel J, Patel A, Patel M, Vyas G (2022) Introduction to nanoparticulate drug delivery systems. Pharmacokinet Pharmacodyn Nanoparticulate Drug Deliv Syst 3–23. https://doi.org/10.1007/978-3-030-83395-4_1
Peternel Š (2013) Bacterial cell disruption: a crucial step in protein production. New Biotechnol 30(2):250–254. https://doi.org/10.1016/J.NBT.2011.09.005
Pietroiusti A, Stockmann-Juvala H, Lucaroni F, Savolainen K (2018) Nanomaterial exposure, toxicity, and impact on human health. Wiley Interdiscip Rev Nanomedicine Nanobiotechnology 10(5). https://doi.org/10.1002/WNAN.1513
Qi G, Li L, Yu F, Wang H (2013) Vancomycin-modified mesoporous silica nanoparticles for selective recognition and killing of pathogenic gram-positive bacteria over macrophage-like cells. ACS Appl Mater Interfaces 5(21):10874–10881. https://doi.org/10.1021/AM403940D
Rippel RA, Seifalian AM (2011) Gold revolution–gold nanoparticles for modern medicine and surgery. J Nanosci Nanotechnol 11(5):3740–3748. https://doi.org/10.1166/JNN.2011.4170
Roduner E (2006) Size matters: why nanomaterials are different. Chem Soc Rev 35(7):583–592. https://doi.org/10.1039/B502142C
Rosario-Meléndez R, Harris CL, Delgado-Rivera R, Yu L, Uhrich KE (2012) PolyMorphine: an innovative biodegradable polymer drug for extended pain relief. J Control Release: off J Control Release Soc 162(3):538–544. https://doi.org/10.1016/J.JCONREL.2012.07.033
Sakmann B, Neher E (1984) Patch clamp techniques for studying ionic channels in excitable membranes. Annu Rev Physiol 46:455–472. https://doi.org/10.1146/ANNUREV.PH.46.030184.002323
Salazar O, Asenjo JA (2007) Enzymatic lysis of microbial cells. Biotech Lett 29(7):985–994. https://doi.org/10.1007/S10529-007-9345-2
Schütte H, Kroner KH, Hustedt H, Kula MR (1983) Experiences with a 20 litre industrial bead mill for the disruption of microorganisms. Enzyme Microb Technol 5(2):143–148. https://doi.org/10.1016/0141-0229(83)90050-9
Silhavy TJ, Kahne D, Walker S (2010) The bacterial cell envelope. Cold Spring Harb Perspect Biol 2(5). https://doi.org/10.1101/CSHPERSPECT.A000414
Singh YD, Ningthoujam R, Panda MK, Jena B, Babu PJ, Mishra AK (2021) Insight from nanomaterials and nanotechnology towards COVID-19. Sens Int 2:100099. https://doi.org/10.1016/J.SINTL.2021.100099
Srikanth M, Kessler JA (2012) Nanotechnology-novel therapeutics for CNS disorders. Nat Rev Neurol 8(6):307–318. https://doi.org/10.1038/NRNEUROL.2012.76
Stackebrandt E, Goodfellow M (1991) Nucleic acid techniques in bacterial systematics. Nucl Acid Tech Bact Syst 115–175. https://doi.org/10.3/JQUERY-UI.JS
Sun-Waterhouse D, Waterhouse GIN (2016) Recent advances in the application of nanomaterials and nanotechnology in food research. Nov Approaches Nanotechnol Food 21–66. https://doi.org/10.1016/B978-0-12-804308-0.00002-9
Sun CQ (2007) Size dependence of nanostructures: impact of bond order deficiency. Prog Solid State Chem 35(1):1–159. https://doi.org/10.1016/J.PROGSOLIDSTCHEM.2006.03.001
Svec D, Andersson D, Pekny M, Sjöback R, Kubista M, Ståhlberg A (2013). Direct cell lysis for single-cell gene expression profiling. Front Oncol 274. https://doi.org/10.3389/FONC.2013.00274/ABSTRACT
Tamura K, Aotsuka T (1988) Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure. Biochem Genet 26(11–12):815–819. https://doi.org/10.1007/BF02395525
Taskova RM, Zorn H, Krings U, Bouws H, Berger RG (2006) A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp. Zeitschrift Fur Naturforschung. C, J Biosci 61(5–6):347–350. https://doi.org/10.1515/ZNC-2006-5-608
Van Den Berg A, Craighead H, Yang P, Mark D, Haeberle S, Gu¨nter A, Roth G, Felix Von Stettenz A, Zengerlez R (2010) Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chem Soc Rev 39(3):1153–1182. https://doi.org/10.1039/B820557B
Vellai T, Vida G (1999) The origin of eukaryotes: the difference between prokaryotic and eukaryotic cells. Proc R Soc B: Biol Sci 266(1428):1571. https://doi.org/10.1098/RSPB.1999.0817
Verma A, Uzun O, Hu Y, Hu Y, Han HS, Watson N, Chen S, Irvine DJ, Stellacci F (2008) Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. Nat Mater 7(7):588–595. https://doi.org/10.1038/NMAT2202
Weiss C, Carriere M, Fusco L, Fusco L, Capua I, Regla-Nava JA, Pasquali M, Pasquali M, Pasquali M, Scott JA, Vitale F, Vitale F, Unal MA, Mattevi C, Bedognetti D, Merkoçi A, Merkoçi A, Tasciotti E, Tasciotti E et al (2020) Toward nanotechnology-enabled approaches against the COVID-19 pandemic. ACS Nano 14(6):6383–6406. https://doi.org/10.1021/ACSNANO.0C03697
Xu ZP, Niebert M, Porazik K, Walker TL, Cooper HM, Middelberg APJ, Gray PP, Bartlett PF, Lu GQ (Max) (2008) Subcellular compartment targeting of layered double hydroxide nanoparticles. J Control Release: Off J Control Release Soc 130(1):86–94. https://doi.org/10.1016/J.JCONREL.2008.05.021
Yang NJ, Hinner MJ (2015) Getting across the cell membrane: an overview for small molecules, peptides, and proteins. Methods Mol Biol (Clifton, N.J.) 1266:29–53. https://doi.org/10.1007/978-1-4939-2272-7_3
Zhang ZY, Smith BD (2000) High-generation polycationic dendrimers are unusually effective at disrupting anionic vesicles: membrane bending model. Bioconjug Chem 11(6):805–814. https://doi.org/10.1021/BC000018Z
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Raghav, A., Kaur, S., Setia, G., Kumar, S. (2024). “Nanomaterials Induced Cell Disruption: An Insight into Mechanism”. In: Shah, M.P., Bharadvaja, N., Kumar, L. (eds) Biogenic Nanomaterials for Environmental Sustainability: Principles, Practices, and Opportunities. Environmental Science and Engineering. Springer, Cham. https://doi.org/10.1007/978-3-031-45956-6_9
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