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Antibacterial Effect of Injectable Platelet-Rich Fibrin Against Periodontal Pathogens

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9th International Conference on the Development of Biomedical Engineering in Vietnam (BME 2022)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 95))

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Abstract

This study aimed to compare the antimicrobial activity of injectable platelet-rich fibrin (i-PRF) obtained from subjects with or without periodontal diseases against two pathogenic bacteria, Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg). I-PRF was prepared from blood samples of 60 individuals, including healthy subjects (n = 20), patients with gingivitis (n = 20), and patients with periodontitis (n = 20). The in vitro antibacterial effect of this platelet concentrate was evaluated using the agar diffusion test, a minimum inhibitory concentration (MIC) assay, and an antibacterial adhesion experiment. I-PRF exhibited a significantly better antibacterial effect against Pg than Aa within the periodontitis group, with a more expansive zone of inhibition and a lower MIC. Among the studied groups, i-PRF collected from the periodontitis group inhibited Aa and Pg significantly more in the agar diffusion test and had a lower MIC than i-PRF collected from the gingivitis and healthy groups. Although i-PRF reduced the adhesion of Aa and Pg and thus their ability to form a biofilm, the difference between groups of patients and the two pathogens was not significant. In conclusion, i-PRF exhibited an antibacterial effect against Aa and Pg in the zone of inhibition, MIC, and biofilm formation inhibition tests. However, in the periodontitis groups, i-PRF exhibited better antibacterial activity against Pg than Aa.

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Acknowledgments

This study was supported by Vietnam National University, Ho Chi Minh City, Vietnam, under grant number: 107/QĐ-ĐHQG (B2021-44-01).

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Authors and Affiliations

  1. Division of Odonto-Stomatology, School of Medicine, Vietnam National University, Ho Chi Minh City, Vietnam

    Thuy Anh Vu Pham

  2. Faculty of Odonto-Stomatology, Hong Bang International University, Ho Chi Minh City, Vietnam

    Thao Tran Thi Phuong

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  1. Thuy Anh Vu Pham
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Correspondence to Thuy Anh Vu Pham .

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Editors and Affiliations

  1. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Van Toi Vo

  2. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Thi-Hiep Nguyen

  3. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Binh Long Vong

  4. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Ngoc Bich Le

  5. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Thanh Qua Nguyen

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Pham, T.A.V., Phuong, T.T.T. (2024). Antibacterial Effect of Injectable Platelet-Rich Fibrin Against Periodontal Pathogens. In: Vo, V.T., Nguyen, TH., Vong, B.L., Le, N.B., Nguyen, T.Q. (eds) 9th International Conference on the Development of Biomedical Engineering in Vietnam. BME 2022. IFMBE Proceedings, vol 95. Springer, Cham. https://doi.org/10.1007/978-3-031-44630-6_76

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  • DOI: https://doi.org/10.1007/978-3-031-44630-6_76

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