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Application of Molecular Markers on Assessing Genetic Diversity in Faba Bean

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Advanced Crop Improvement, Volume 2

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Abstract

With the advancement of sequencing technologies, the identification of molecular approaches has been used for the preservation of gene pool sources and for improving breeding programs in recent years. At the molecular level, diversity within and between genotypes or accessions is commonly assessed using various laboratory-based techniques, such as allozyme or DNA analysis, which directly determine diversity levels. Population size, heterozygosity, and quantification of genetic diversity are employed as fitness indicators and positively correlated with population fitness. The current study provides an overview and application of the molecular marker techniques used for the analysis of genetic diversity in faba beans (Vicia faba L.). Different markers were used to identify specific genes, gene actions and construct gene maps that helped in the development of gene transfer. Furthermore, these markers have also played a significant role to determine the species evolution and phylogeny analysis and help to a better understanding of the distribution and breadth of the genetic diversity within and among the species. The current chapter discussed the application of different molecular markers, i.e., non-PCR-based markers (RFLP), PCR-based markers (RAPD, ISSR, SSR, SSAP, AFLP, SRAP), and sequence-based markers (SNP) that were used for the improvement of faba bean. The advanced marker technique has an advantage over the basic technique and enhanced resolution and sensitivity to detect the genetic gap and differences among the genotypes.

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Authors and Affiliations

  1. Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia

    Nurmansyah

  2. Production Department, Faculty of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia

    Salem S. Alghamdi & M. Afzal

  3. National Agricultural Research Center, Baqa, Jordan

    Hussein M. Migdadi

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  1. Nurmansyah
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  2. Salem S. Alghamdi
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  3. Hussein M. Migdadi
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  4. M. Afzal
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Corresponding author

Correspondence to Nurmansyah .

Editor information

Editors and Affiliations

  1. Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Aamir Raina

  2. Department of Botany, Abdul Ahad Azad Memorial Degree College Bemina, Cluster University, Srinagar, Jammu and Kashmir, India

    Mohammad Rafiq Wani

  3. Department of Botany, Pandit Deendayal Upadhyaya Adarsha Mahavidyalaya (PDUAM), Eraligool, Karimganj, Assam, India

    Rafiul Amin Laskar

  4. Molecular Biology Laboratory, Maritsa Vegetable Crops Research Institute, Agricultural Academy, Plovdiv, Bulgaria

    Nasya Tomlekova

  5. Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India

    Samiullah Khan

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Nurmansyah, Alghamdi, S.S., Migdadi, H.M., Afzal, M. (2023). Application of Molecular Markers on Assessing Genetic Diversity in Faba Bean. In: Raina, A., Wani, M.R., Laskar, R.A., Tomlekova, N., Khan, S. (eds) Advanced Crop Improvement, Volume 2. Springer, Cham. https://doi.org/10.1007/978-3-031-26669-0_11

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