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Lung-on-a-Chip Models of the Lung Parenchyma

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Engineering Translational Models of Lung Homeostasis and Disease

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1413))

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Abstract

Since the publication of the first lung-on-a-chip in 2010, research has made tremendous progress in mimicking the cellular environment of healthy and diseased alveoli. As the first lung-on-a-chip products have recently reached the market, innovative solutions to even better mimic the alveolar barrier are paving the way for the next generation lung-on-chips. The original polymeric membranes made of PDMS are being replaced by hydrogel membranes made of proteins from the lung extracellular matrix, whose chemical and physical properties exceed those of the original membranes. Other aspects of the alveolar environment are replicated, such as the size of the alveoli, their three-dimensional structure, and their arrangement. By tuning the properties of this environment, the phenotype of alveolar cells can be tuned, and the functions of the air-blood barrier can be reproduced, allowing complex biological processes to be mimicked. Lung-on-a-chip technologies also provide the possibility of obtaining biological information that was not possible with conventional in vitro systems. Pulmonary edema leaking through a damaged alveolar barrier and barrier stiffening due to excessive accumulation of extracellular matrix proteins can now be reproduced. Provided that the challenges of this young technology are overcome, there is no doubt that many application areas will benefit greatly.

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Acknowledgements

This work was supported by funding from the Swiss National Science Foundation (project: 185365), the Swiss 3R Competence Centre (project: 3RCC-OC-2019-025), the H2020 Eurostars (project: AIM4DoC) and MSCA-ITN (EUROoC, project: 812954), Innosuisse (project: 48818.1 IP-LS) and the Novartis Foundation for medical-biological Research (project: #20B146). The authors thank Dr. Anne Morbach for the illustration of Fig. 10.2.

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Author notes

  1. Pauline Zamprogno and Jan Schulte contributed equally.

Authors and Affiliations

  1. Organs-on-Chip Technologies Laboratory, ARTORG Center, University of Bern, Bern, Switzerland

    Pauline Zamprogno, Jan Schulte, Dario Ferrari, Karin Rechberger, Arunima Sengupta, Lisette van Os, Tobias Weber, Soheila Zeinali & Olivier T. Guenat

  2. Department of Pulmonary Medicine, University Hospital of Bern, Bern, Switzerland

    Thomas Geiser & Olivier T. Guenat

  3. Department of General Thoracic Surgery, University Hospital of Bern, Bern, Switzerland

    Olivier T. Guenat

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Correspondence to Olivier T. Guenat .

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  1. University of Colorado, Denver | Anschutz Medical Campus, Aurora, CO, USA

    Chelsea M. Magin

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Zamprogno, P. et al. (2023). Lung-on-a-Chip Models of the Lung Parenchyma. In: Magin, C.M. (eds) Engineering Translational Models of Lung Homeostasis and Disease. Advances in Experimental Medicine and Biology, vol 1413. Springer, Cham. https://doi.org/10.1007/978-3-031-26625-6_10

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