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NADPH Oxidase-Dependent Processes in the Social Amoeba Dictyostelium discoideum

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NADPH Oxidases Revisited: From Function to Structure

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Abstract

Dictyostelium discoideum, also known as social amoeba, is at the boundary between unicellular and multicellular life. This protist is a professional phagocyte in its vegetative unicellular stage, feeding on bacterial preys, and it enters a multicellular phase in response to starvation. Dictyostelium and human phagocytic cells share several unique functions and this similarity is supported by a high degree of conservation between their proteomes. Three homologs of the large subunit of NADPH oxidase (NoxA, B, C), a homolog of the small subunit p22phox and a p67phox-like factor have been identified. In this chapter, roles involving Dictyostelium NADPH oxidase activities are described: development and cell differentiation, intraphagosomal bacterial killing and formation of DNA-based extracellular traps by sentinel cells, a subtype of amoebal cells that support ancestral innate immunity.

The exploration of NOX functions and regulation is still ongoing, and their study in alternative models is needed for a comprehensive and integrated view of the contribution of NADPH oxidases to key biological processes. In this context, the amoeba Dictyostelium is a particularly attractive model to enrich our current understanding of this family of enzymes.

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Authors and Affiliations

  1. University Grenoble Alpes, CEA, INSERM, IRIG, BGE, Grenoble, France

    Laurence Aubry

  2. University Grenoble Alpes, TIMC, UMR 5525 CNRS, GREPI, Grenoble, France

    Bernard Lardy

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  1. Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

    Edgar Pick

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Aubry, L., Lardy, B. (2023). NADPH Oxidase-Dependent Processes in the Social Amoeba Dictyostelium discoideum. In: Pick, E. (eds) NADPH Oxidases Revisited: From Function to Structure. Springer, Cham. https://doi.org/10.1007/978-3-031-23752-2_23

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