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Tailoring the Microenvironment of Cells Towards Osteogenic Differentiation Using Multilayers of Glycosaminoglycans and Growth Factor Immobilization

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Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications

Part of the book series: Engineering Materials ((ENG.MAT.))

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Abstract

The extracellular matrix (ECM) provides the structural support and signals important for cell fate, tissue homeostasis and regeneration. Layer-by-Layer technique can apply ECM components like glycosaminoglycans (GAGs) and proteins due to their intrinsic charge to make ECM-like multilayers. Here, the effect of microenvironment on controlling human adipose-derived stem cells’ (hADSCs) behavior was studied using multilayers composed of native GAGs (nGAGs) such as, hyaluronic acid (HA) and chondroitin sulfate (CS) or their oxidized forms “oGAGs” (oHA & oCS) in combination with collagen I; based primarily on ion pairing and on additional intrinsic covalent cross-linking by imine bond formation. Osteogenic differentiation of hADSCs was found to be stronger, when collagen I was combined with CS and in case of intrinsic cross-linking using oCS. While these studies still required osteogenic media to achieve differentiation, the second part of this study was conducted using heparin (H) and CS that were combined with chitosan as polycation. oH and oCS permitted not only intrinsic cross-linking to enhance the multilayer stability, but also controlled the release of the osteogenic growth factor BMP-2. There, the oGAGs systems were superior to nGAGs in controlling osteogenic differentiation of C2C12 muscle cells. Overall, multilayers made of oxidized CS with either collagen I or chitosan as polycations, seem to be useful to direct cell differentiation towards osteogenic and other lineages

Reema Anouz and Mingyan Zhao are contributed equally.

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Acknowledgements

The financial support to R.A by the International Graduate School AGRIPOLY supported by the European Regional Development Fund (ERDF) and the Federal State Saxony-Anhalt is greatly acknowledged. We are also thankful for the financial support that was also provided by a grant form Deutsche Forschungsgemeinschaft to TG (Gr1290/11-1). The National Natural Science Foundation of China (32071326), the Natural Science Foundation of Guangdong Province, China (2019A1515011613, 2021A1515011196), the Zhanjiang competitive funding project, China (2018A01032, 2020A01018) and the Characteristic innovation projects of Guangdong Province universities (2018KTSCX076).

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Authors and Affiliations

  1. Biomedical Materials Department, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120, Halle, Saale, Germany

    Reema Anouz & Thomas Groth

  2. Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China

    Mingyan Zhao & Fan Gong

  3. Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China

    Fan Gong

  4. Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, Heinrich-Damerow Strasse 4, 06120, Halle, Saale, Germany

    Thomas Groth

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  1. Reema Anouz
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  4. Thomas Groth
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  1. Faculty of Medicine, University of Niš, Niš, Serbia

    Stevo Najman

  2. (Deceased), Niš, Serbia

    Vojislav Mitić

  3. Department of Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

    Thomas Groth

  4. BerlinAnalytix GmbH, Berlin, Germany

    Mike Barbeck

  5. Department of Materials Science and Engineering, National Tsing Hua University, Hsinchu, Taiwan

    Po-Yu Chen

  6. School of Chemistry and Physics, Queensland University of Technology, Brisbane, QLD, Australia

    Ziqi Sun

  7. Department of Mathematics, Faculty of Electronic Engineering, University of Niš, Niš, Serbia

    Branislav Randjelović

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Anouz, R., Zhao, M., Gong, F., Groth, T. (2023). Tailoring the Microenvironment of Cells Towards Osteogenic Differentiation Using Multilayers of Glycosaminoglycans and Growth Factor Immobilization. In: Najman, S., et al. Bioceramics, Biomimetic and Other Compatible Materials Features for Medical Applications. Engineering Materials. Springer, Cham. https://doi.org/10.1007/978-3-031-17269-4_1

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