Abstract
Mushrooms, generally belonging to Basidiomycetes, harbor numerous metabolites of nutraceutical and therapeutic significance. Since ancient civilizations, medicinal and edible mushrooms have been used by humans not only as valued food but also in medications. Mushrooms are manufacturers of diverse secondary metabolites possessing more than 130 therapeutic properties including antibacterial, antiviral, analgesic, antifungal, antioxidant, anti-inflammatory, antiplatelet, hepatoprotective, hypoglycemic, immunomodulatory, hypocholesterolemic, mitogenic/regenerative, and many more. Recently, researches have advanced in the area of exploration of these valuable low-molecular-weight metabolites to discover novel bioactive compounds. These mushroom derived compounds have tremendous potential for sustainable agriculture and in industrial sectors, also. This chapter deals with the protocol for extraction, isolation, and purification of valuable functional secondary metabolites of mushrooms. A wide range of bioactive compounds derived from Ganoderma spp., Lentinula edodes, and Flammulina velutipe have been discussed, along with their biological activities of direct applications in pharmaceutical, nutraceuticals manufacturing, or any biological management program.
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References
Afrin, S., Rakib, M. A., Kim, B. H., Kim, J. O., & Ha, Y. L. (2016). Eritadenine from edible mushrooms inhibits activity of angiotensin converting enzyme in vitro. Journal of Agricultural and Food Chemistry, 64, 2263–2268.
Baby, S., Johnson, A. J., & Govindan, B. (2015). Secondary metabolites from Ganoderma. Phytochemistry, 114, 66–101.
Béni, Z., Dékány, M., Kovács, B., Csupor-Löffler, B., Zomborszki, Z. P., Kerekes, E., Szekeres, A., Urbán, E., Hohmann, J., & Ványolós, A. (2018). Bioactivity-guided isolation of antimicrobial and antioxidant metabolites from the mushroom Tapinella atrotomentosa. Molecules: A Journal of Synthetic Chemistry and Natural Product Chemistry, 23, 1082.
Bérdy, J. (2012). Thoughts and facts about antibiotics: Where we are now and where we are heading. The Journal of Antibiotics, 65, 385–395.
Boh, B., Hodzar, D., Dolnicar, D., Berovic, M., & Pohleven, F. (2000). Isolation and quantification of triterpenoid acids from Ganoderma applanatum of Istrianorigin. Food Technology and Biotechnology, 38, 11–18.
Boonsong, S., Klaypradit, W., & Wilaipun, P. (2016). Antioxidant activities of extracts from five edible mushrooms using different extractants. Agriculture and Natural Resources, 50, 89–97.
Bubalo, C. M., Vidović, S., Redovniković, I. R., & Jokić, S. (2018). New perspective in extraction of plant biologically active compounds by green solvents. Food and Bioproducts Processing, 109(52–73), 109. https://doi.org/10.1016/j.fbp.2018.03.001
Cantley, M. F. (1997). International instruments, intellectual property and collaborative exploitation of genetic resources. In S. Wrigley (Ed.), Phytochemical diversity: A source of new industrial products (pp. 141–157). Royal Society of Chemistry.
Chairul, S. M., & Hayashi, Y. (1994). Lanostanoid triterpenes from Ganoderma applanatum. Phytochemistry, 35, 1305–1308.
Chen, J., Wei, S. L., & Gao, K. (2015). Chemical constituents and antibacterial activities of compounds from Lentinus edodes. Chemistry of Natural Compounds, 51, 592–594.
Chen, S., Xu, J., Liu, C., Zhu, Y., Nelson, D. R., Zhou, S., Li, C., Wang, L., Guo, X., Sun, Y., Luo, H., Li, Y., Song, J., Henrissat, B., Levasseur, A., Qian, J., Li, J., Luo, X., Shi, L., … Sun, C. (2012). Genome sequence of the model medicinal mushroom Ganoderma lucidum. Nature Communications, 3, 913.
Cheng, C. R., Yue, Q. X., Wu, Z. Y., Song, X. Y., Tao, S. J., Wu, X. H., Xu, P. P., Liu, X., Guan, S. H., & Guo, D. A. (2010). Cytotoxic triterpenoids from Ganoderma lucidum. Phytochemistry, 71, 1579–1585.
Dembitsk, V. M., & Maoka, T. (2007). Allenic and cumulenic lipids. Progress in Lipid Research, 46, 328–375.
Fatmawati, S., Shimizu, K., & Kondo, R. (2010). Ganoderic acid Df, a new triterpenoid with aldose reductase activity from the fruiting body of Ganoderma lucidum. Fitoterapia, 81, 1033–1036.
Feeney, M. J., Miller, A. M., & Roupas, P. (2014). Mushrooms – Biologically distinct and nutritionally unique: Exploring a “third food kingdom”. Nutrition Today, 49(6), 301–307.
Finimundy, T. C., Dillon, A. J. P., Henriques, J. A. P., & Ely, M. R. (2014). A review on general nutritional compounds and pharmacological properties of the Lentinula edodes mushroom. Food and Nutrition Sciences, 5, 1095–1105.
Fukushima-Sakuno, E. (2020). Bioactive small secondary metabolites from the mushrooms Lentinula edodes and Flammulina velutipes. The Journal of Antibiotics, 73. https://doi.org/10.1038/s41429-020-0354-x
Gargano, M. L., van Griensven, L. J. L. D., Isikhuemhen, O. S., Lindequist, U., Venturella, G., Wasser, S. P., & Zervakis, G. I. (2017). Medicinal mushrooms: Valuable biological resources of high exploitation potential. Plant Biosystems, 151, 548–565.
Hashim, S. N. N. S., Schwarz, L. J., Danylec, B., Mitri, K., Yang, Y., Boysen, R. I., & Hearn, M. T. W. (2016). Recovery of ergosterol from the medicinal mushroom, Ganoderma tsugae, var. Janniae, with a molecularly imprinted polymer derived from a cleavable monomer-template composite. Journal of Chromatography A, 1468, 1–9.
Hassainia, A., Satha, H., & Boufi, S. (2018). Chitin from Agaricus bisporus: Extraction and characterization. International Journal of Biological Macromolecules, 117, 1334–1342.
Herrmann, H. (1962). Cortinellin, eine antibiotisch wirkasame Substanz aus Cortinellus shiitake. Naturwissenschaften, 49, 542.
Hirai, Y., Ikeda, M., Murayama, T., & Ohata, T. (1998). New monoterpentriols from the fruiting body of Flammulina velutipes. Bioscience, Biotechnology, and Biochemistry, 62, 1364–1368.
Isaka, M., Chinthanom, P., Kongthong, S., Srichomthong, K., & Choeyklin, R. (2013). Lanostane triterpenes from cultures of the basidiomycete Ganoderma orbiforme BCC 22324. Phytochemistry, 87, 133–139.
Kaewnarin, K., Suwannarach, N., Kumla, J., & Lumyong, S. (2016). Phenolic profile of various wild edible mushroom extracts from Thailand and their antioxidant properties, anti-tyrosinase and hyperglycaemic inhibitory activities. Journal of Functional Foods, 27, 352–364.
Kała, K., Kryczyk-Poprawa, A., Rzewińska, A., & Muszyńska, B. (2020). Fruiting bodies of selected edible mushrooms as a potential source of lovastatin. European Food Research and Technology, 246, 713–722.
Keswani, C., Mishra, S., Sarma, B. K., Singh, S. P., & Singh, H. B. (2014). Unravelling the efficient applications of secondary metabolites of various Trichoderma spp. Applied Microbiology and Biotechnology, 98, 533–544.
Kimatu, B. M., Zhao, L., Biao, Y., Ma, G., Yang, W., Pei, F., & Hu, Q. (2017). Antioxidant potential of edible mushroom (Agaricus bisporus) protein hydrolysates and their ultrafiltration fractions. Food Chemistry, 230, 58–67.
Komemushi, S., Yamamoto, Y., & Fujita, T. (1996). Purification and identification of antimicrobial substances produced by Lentinus edodes. Journal of Antibacterial and Antifungal Agents, 24, 21–25.
Kumagai, H., Akao, M., & Masuda, H. (2013). Hepatopathy inhibitor. Japan Kokai Tokkyo Koho, JP2013103900A.
Li, Y.-Y., Mi, Z.-Y., Tang, Y., Wang, G., Li, D.-S., & Tang, Y. J. (2009). Lanostanoids isolated from Ganoderma lucidum mycelium cultured by submerged fermentation. Helvetica Chimica Acta, 92, 1586–1593.
Lin, S., Chen, Y., Yu, H., Barseghyan, G. S., Asatiani, M. D., & Wasser, S. P. (2013). Comparative study of contents of several bioactive components in fruiting bodies and mycelia of culinary-medicinal mushrooms. International Journal of Medicinal Mushrooms, 15, 315–323.
Liu, L. Y., Chen, H., Liu, C., Wang, H. Q., Kang, J., Li, Y., & Chen, R. Y. (2014). Triterpenoids of Ganoderma theaecolum and their hepatoprotective activities. Fitoterapia, 98, 254–259.
Maeng, J. H., Muhammad Shahbaz, H., Ameer, K., Jo, Y., & Kwon, J. H. (2016). Optimization of microwave-assisted extraction of bioactive compounds from Coriolus versicolor mushroom using response surface methodology. Journal of Food Process Engineering, 40. https://doi.org/10.1111/jfpe.12421
Money, N. P. (2016). Are mushrooms medicinal? Fungal Biology, 120, 449–453.
Morales, D., Piris, A. J., Ruiz-Rodriguez, A., Prodanov, M., & Soler-Rivas, C. (2018). Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method. Biotechnology Progress, 34(3), 746–755.
Morita, K., & Kobayashi, S. (1967). Isolation, structure, and synthesis of lenthionine and its analogs. Chemical & Pharmaceutical Bulletin, 15, 988–993.
Raut, J. K. (2019). Current status, challenges and prospects of mushroom industry in Nepal. International Journal of Agricultural Economics, 4, 154–160.
Ríos, J. L., Andújar, I., Recio, M. C., & Giner, R. M. (2012). Lanostanoids from fungi: A group of potential anticancer compounds. Journal of Natural Products, 75, 2016–2044.
Roselló-Soto, E., Parniakov, O., Deng, Q., Patras, A., Koubaa, M., Grimi, N., & Barba, F. J. (2016). Application of non-conventional extraction methods: Toward a sustainable and green production of valuable compounds from mushrooms. Food Engineering Reviews, 8(2), 214–234.
Royse, D. J., Baars, J., & Tan, Q. (2017). Current overview of mushroom production in the world. In D. C. Zied & Pardo-Giménez (Eds.), Edible and medicinal mushrooms: Technology and applications (pp. 5–13). Wiley.
Ruthes, A. C., Smiderle, F. R., & Iacomini, M. (2015). D-glucans from edible mushrooms: A review on the extraction, purification and chemical characterization approaches. Carbohydrate Polymers, 117, 753–761.
Sanodiya, B. S., Thakur, G. S., Baghel, R. K., Prasad, G. B., & Bisen, P. S. (2009). Ganoderma lucidum: A potent pharmacological macrofungus. Current Pharmaceutical Biotechnology, 10, 717–742.
Sato, N., Zhang, Q., Ma, C. M., & Hattori, M. (2009). Anti-human immunodeficiency virus-1 protease activity of new lanostane-type triterpenoids from Ganoderma sinense. Chemical & Pharmaceutical Bulletin, 57, 1076–1080.
Seo, H. W., Hung, T. M., Na, M., Jung, H. J., Kim, J. C., Choi, J. S., Kim, J. H., Lee, H. K., Lee, I., Bae, K., Hattori, M., & Min, B. S. (2009). Steroids and triterpenes from the fruit bodies of Ganoderma lucidum and their anti-complement activity. Archives of Pharmacal Research, 32, 1573–1579.
Smania, A., Jr., Monache, F. D., Smania, E. F. A., & Cuneo, R. S. (1999). Antibacterial activity of steroidal compounds isolated from Ganoderma applanatum (Pers.) Pat. (Aphyllophoromycetideae) fruit body. International Journal of Medicinal Mushrooms, 1, 325–330.
Smiderle, F. R., Morales, D., Gil-Ramírez, A., de Jesus, L. I., Gilbert-López, B., Iacomini, M., & Soler-Rivas, C. (2017). Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms. Carbohydrate Polymers, 156, 165–174.
Stadler, M., Hellwig, V., Mayer-Bartschmid, A., Denzer, D., Wiese, B., & Burkhardt, N. (2006). Novel analgesic triglycerides from cultures of Agaricus macrosporus and other basidiomycetes as selective inhibitors of neurolysin. The Journal of Antibiotics, 58, 775–786. https://doi.org/10.1038/ja.2005.105
Stadler, M., & Hoffmeister, D. (2015). Fungal natural products—the mushroom perspective. Frontiers in microbiology, 6, 127.
Tabuchi, A., Fukushima-Sakuno, E., Osaki-Oka, K., Futamura, Y., Motoyama, T., Osada, H., et al. (2020). Productivity and bioactivity of enokipodins A–D of Flammulina rossica and Flammulina velutipes. Bioscience, Biotechnology, and Biochemistry, 84, 876–886.
Tang, C., Hoo, P. C., Tan, L. T., Pusparajah, P., Khan, T. M., Lee, L., et al. (2016). Golden needle mushroom: A culinary medicine with evidenced based biological activities and health promoting properties. Frontiers in Pharmacology, 7, Article 474.
Tao, Q., Ma, K., Yang, Y., Wang, K., Chen, B., Huang, Y., et al. (2016). Bioactive sesquiterpenes from the edible mushroom Flammulina velutipes and their biosynthetic pathway confirmed by genome analysis and chemical evidence. The Journal of Organic Chemistry, 81(98), 67–77.
Tokimoto, K., & Komatsu, M. (1995). Selection and breeding of Shiitake strains resistant to Trichoderma spp. Canadian Journal of Botany, 73(S1), 962–966.
Wang, F., & Liu, J. K. (2008). Highly oxygenated lanostane triterpenoids from the fungus Ganoderma applanatum. Chemical & Pharmaceutical Bulletin, 56, 1035–1037.
Wang, Y., Bao, L., Yang, X., Li, L., Li, S., Gao, H., et al. (2012). Bioactive sesquiterpenoids from the solid culture of the edible mushroom Flammulina velutipes growing on cooked rice. Food Chemistry, 132, 1346–1353.
Xu, Z. Y., Wu, Z. A., & Bi, K. S. (2013). A novel norsesquiterpene alkaloid from the mushroom-forming fungus Flammulina velutipes. Chinese Chemical Letters, 24, 57–58.
Yang, M., Wang, X. M., Guan, S. H., **a, J. M., Sun, J. H., Guo, H., & Guo, D. A. (2007). Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, 18, 927–939.
Yang, S. X., Yu, Z. C., Lu, Q. Q., Shi, W. Q., Laatsch, H., & Gao, J. M. (2012). Toxic lanostane triterpenes from the basidiomycete Ganoderma amboinense. Phytochemistry Letters, 5, 576–580.
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Afzal, K., Shukla, A.C., Srivastava, D.K. (2022). Protocols for Extraction, Isolation, and Purification of Secondary Metabolites of Mushroom and Its Applications. In: Shukla, A.C. (eds) Applied Mycology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-90649-8_8
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DOI: https://doi.org/10.1007/978-3-030-90649-8_8
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