Hydration of Cells and Tissues

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Biological Water

Part of the book series: Soft and Biological Matter ((SOBIMA))

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Abstract

Biological cells consist of cytoplasm, which contains many biomolecules such as proteins and nucleic acids, enclosed within a membrane. Tissues are composed of anywhere from 20 to 80% water by volume depending on the type of tissue and the location within the tissue. The chapter describes the molecular status of water in cancer, red blood cells, Escherichia coli, Haloarcula marismortui and miscellaneous cells. Picosecond vibrational spectroscopy, dielectric-relaxation spectroscopy, confocal Raman microspectroscopy and other techniques were employed to observe the random orientational motion of water molecules inside living cells. Separate sections are dedicated to the water inside mammalian, food, and plants tissues.

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References

  1. G. Cooper, Cell: A Molecular Approach (ASM PRESS, Washington, DC, 2009), p. 544. ISBN 978-0-87893-300-6

    Google Scholar 

  2. G. de Majno, I. Joris, Cells, Tissues, and Disease: Principles of General Pathology (Oxford University Press, Oxford, 2004)

    Google Scholar 

  3. G. Vunjak-Novakovic, R.I. Freshney, Culture of Cells for Tissue Engineering (Wiley, Hoboken, 2006)

    Google Scholar 

  4. P. Balla, Water is an active matrix of life for cell and molecular biology. PNAS 114, 13327–13335 (2017)

    Article  Google Scholar 

  5. J.M. Carbrey, P. Agre, Discovery of the aquaporins and development of the field. Handb. Exp. Pharmacol. 190, 3–28 (2009)

    Article  Google Scholar 

  6. P. Jungwirth, Biological water or rather water in biology? J. Phys. Chem. Lett. 6, 2449–2451 (2015)

    Article  Google Scholar 

  7. M.L. Martins, A.B. Dinitzen, E. Mamontov, S. Rudić, J.E.M. Pereira, R. Hartmann-Petersen, W. Herwig, H.N. Bordallo, Water dynamics in MCF-7 breastcancer cells: a neutron scattering descriptive study. Sci. Rep. 9, 8704 (2019)

    Google Scholar 

  8. C.Y.J. Hémonnot, S. Köster, Imaging of biological materials and cells by X-ray scattering and diffraction. ACS Nano 11, 8542–8559 (2017)

    Article  Google Scholar 

  9. M. Bartels, M. Krenkel, J.R. Haber, R. Wilke, T. Salditt, X-ray holographic imaging of hydrated biological cells in solution. Phys. Rev. Lett. 114, 048,103 (2015)

    Google Scholar 

  10. S. Ayrapetyan, The dysfunction of metabolic controlling of cell hydration precedes Warburg phenomenon in carcinogenesis. J. Bioequiv. Availab. 7, 1 (2015)

    Article  Google Scholar 

  11. R. Smith, K.L. Wright, L. Ashton, Raman spectroscopy: an evolving technique for live cell studies. Analyst 141, 3590–3600 (2016)

    Article  ADS  Google Scholar 

  12. E. Persson, B. Halle, Cell water dynamics on multiple time scales. Proc. Natl. Acad. Sci. U.S.A. 105, 6266–6271 (2007)

    Article  ADS  Google Scholar 

  13. L. Shi, F. Hu, W. Min, Optical map** of biological water in single live cells by stimulated Raman excited fluorescence microscopy. Nat. Commun. 10, Article number: 4764 (2019)

    Google Scholar 

  14. S. Sabbatini, C. Conti, G. Orilisi, E. Giorgini, Infrared spectroscopy as a new tool for studying single living cells: Is there a niche? Biomed. Spectrosc. Imaging 6, 85–99 (2017)

    Article  Google Scholar 

  15. P. Ball, Water as an active constituent in cell biology. Chem. Rev. 108, 74–108 (2008)

    Article  Google Scholar 

  16. A. Orecchini, F. Sebastiani, M. Jasnin, A. Paciaroni1, A. De Francesco, C. Petrillo, M. Moulin, M. Haertlein, G. Zaccai, F. Sacchetti, Collective dynamics of intracellular water in living cells. J. Phys.: Conf. Ser. 340, 012091 (2012)

    Google Scholar 

  17. M. Takeuchi, S. Kajimoto, T. Nakabayashi, Experimental evaluation of the density of water in a cell by Raman microscopy. J. Phys. Chem. Lett. 8, 5241–5245 (2017)

    Article  Google Scholar 

  18. L.C. Conceiçao, K. Meehan, M. Antoniassi, M. Piacenti-Silva, M.E. Poletti. The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues. Heliyon 5, e01219 (2019) abnormal

    Google Scholar 

  19. M.I.H. Khan, R.M. Wellard, S.A. Nagy, M.U.H. Joardder, M.A. Karim, Investigation of bound and free water in plant-based food material using NMR T2 relaxometry. Innov. Food Sci. Emerg. Technol. 38, 252–261 (2016)

    Article  Google Scholar 

  20. K. Shiraga, T. Suzuki, N. Kondo, K. Tanaka, K.Y. Ogawa, Hydration state inside HeLa cell monolayer investigated with terahertz spectroscopy. Appl. Phys. Lett. 106, 253701 (2015)

    Google Scholar 

  21. J.M. Dick, Proteomic indicators of oxidation and hydration state in colorectal cancer. Peer J. 4, e2238 (2016)

    Google Scholar 

  22. T.M. Grzegorczyk, P.M. Meaney, P.A. Kaufman, R.M. Di Florio-Alexander, K.D. Paulsen, Fast 3-D tomographic microwave imaging for breast cancer detection. IEEET Trans. Med. Imaging 31, 1584–1592 (2012)

    Google Scholar 

  23. G.I. McIntyre, Cell hydration as the primary factor in carcinogenesis: a unifying concept. Med. Hypotheses 66, 518–526 (2006)

    Article  Google Scholar 

  24. H. Abramczyk, B. Brozek-Pluska, M. Krzesniak, M. Kopec, A. Morawiec-Sztandera. The cellular environment of cancerous human tissue. Interfacial and dangling water as a “hydration fingerprint”. Spectrochimica Acta, Part A: Mol. Biomol. Spectrosc. 129, 609–623 (2014)

    Google Scholar 

  25. M.P.M. Marques, A.L.M. Batista de Carvalho, A.P. Mamede et al., Struct. Dyn. 7, 05470 (2020)

    Google Scholar 

  26. M. Takeuchi, S. Kajimoto, T. Nakabayashi, Experimental evaluation of the density of water in a cell by Raman microscopy. J. Phys. Chem. Lett. 8, 5241–5245 (2017). https://doi.org/10.1021/acs.jpclett.7b02154

    Article  Google Scholar 

  27. R. Minnes, M. Nissinmann, Y. Maizels, G. Gerlitz, A. Katzir, Y. Raichlin, Using attenuated total reflection–Fourier Transform Infra-Red (ATRFTIR) spectroscopy to distinguish between melanoma cells with a different metastatic potential. Sci. Rep. 7, 4381 (2017)

    Article  ADS  Google Scholar 

  28. A. Tomback, Erythrocyte (IntechOPEN 2019)

    Google Scholar 

  29. G.W. Stewart, E.J. Turner, The hereditary stomatocytoses and allied disorders: congenital disorders of erythrocyte membrane permeability to Na and K. Best Pract. Res. Clin. Haematol. 12, 707–727 (1999)

    Article  Google Scholar 

  30. T. Tiffert, V.L. Lew, H. Ginsburg, M. Krugliak, L. Croisille, N. Mohandas, The hydration state of human red blood cells and their susceptibility to invasion by Plasmodium falciparum. Blood 105, 4853–4860 (2005)

    Google Scholar 

  31. A.M. Stadler, J.P. Embs, I. Digel, G.M. Artmann, T. Unruh, Z. Georg Buldt, Cytoplasmic water and hydration layer dynamics in human red blood cells. J. Am. Chem. Soc. 130, 16852–16853 (2008)

    Google Scholar 

  32. P.G. Gallagher, Disorders of erythrocyte hydration. Blood 130, 2699–2708 (2017)

    Article  Google Scholar 

  33. E.R. Brown, W.-D. Zha, The Critical Effect of Hydration on the Resonant Signatures of THz Biospectroscopy. https://www.researchgate.net/publication/328445311

  34. A. Caulier, R. Rapetti-Mauss, H. Guizouarn, V. Picard, L. Garçon, C. Badens, Primary red cell hydration disorders: pathogenesis and diagnosis. Int. J. Lab. Hem. 40, 68–73 (2018)

    Google Scholar 

  35. M. Tehei, B. Franzetti, K. Wood, F. Gabel, E. Fabiani, M. Jasnin, M. Zamponi, D. Oesterhelt, G. Zaccai, M. Ginzburg, B.-Z. Ginzburg, Neutron scattering reveals extremely slow cell water in a Dead Sea organism, PNAS 104, 766–771 (2007)

    Google Scholar 

  36. M. Jasnin, M. Moulin, M. Haertlein, G. Zaccai1, M. Tehei, Down to atomic-scale intracellular water dynamics. EMBO Rep. 9, 543–546 (2008)

    Google Scholar 

  37. A. Orecchini, F. Sebastiani, M. Jasnin, A. Paciaroni, A. De Francesco, C. Petrillo, M. Moulin, M. Haertlein, G. Zaccai, F. Sacchetti, Collective dynamics of intracellular water in living cells. J. Phys.: Conf. Ser. 340, 012091 (2012)

    Google Scholar 

  38. M. Tros, L. Zheng, J. Hunger, M. Bonn, D. Bonn, S. Daniel, S. Gertien, S. Woutersen, Picosecond orientational dynamics of water in living cells. Nat. Commun. 8, 904 (2017)

    Google Scholar 

  39. A. Abazari, N. Chakraborty, S. Hand, A. Aksan, M. Toner, A Raman microspectroscopy study of water and trehalose in spin-dried cells. Biophys. J. 107, 2253–2262 (2014)

    Article  ADS  Google Scholar 

  40. M. Nuriya, H. Yoneyama, K. Takahashi, Characterization of intra/extracellular water states probed by ultrabroadband multiplex coherent anti-stokes Raman scattering (CARS) spectroscopic imaging. J. Phys. Chem. A 123, 3928–3934 (2019)

    Article  Google Scholar 

  41. R. Da Costa, S. Amaral, K. Redmann, S. Kliesch, S. Schlatt, Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: effects of UV-irradiation and hydration. PLOS ONE (2018) https://doi.org/10.1371/journal.pone.0207786

  42. S. Ayrapetyan, J. DeCell, Hydration as a biomarker for estimation of biological effects of nonionizing radiation on cells and organisms. Sci. World J. Article ID 890518, 8 pages (2014)

    Google Scholar 

  43. E.R. Brown, W-D. Zha, The critical effect of hydration on the resonant signatures of THz. Biospectroscopy. https://www.researchgate.net/publication/328445311

  44. Z.D. Taylor, R.S. Singh, D.B. Bennett, P. Tewari, C.P. Kealey, N. Bajwa, M.O. Culjat, A. Stojadinovic, H. Lee, J.-P. Hubschman, E.R. Brown, W.S. Grundfest, THz medical imaging: in vivo hydration sensing. IEEE Trans. Terahertz Sci. Technol. 1(1), 201–219 (2011). https://doi.org/10.1109/TTHZ.2011.2159551

  45. D.B. Bennett, Z.D. Taylor, D. Sassoon, P. Tewari, R.D. Johnson, R. Singh, M.O. Culjat, J-P Hubschman, W.S. Grundfest, D.J. Sassoon, R. Duncan Johnson, J.-P. Hubschman, E.R. Brown, Terahertz sensing in corneal tissues. J. Biomed. Opt. 16, 8 (2010)

    Google Scholar 

  46. A.L.C. Conceiçao, K. Meehan, M. Antoniassi, M. Piacenti-Silva, M.E. Poletti, The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues. Heliyon e01219 (2019)

    Google Scholar 

  47. E. Moser, P. Holzmueller, M. Krssak, Improved estimation of tissue hydration and bound water fraction in rat liver tissue. Magn. Reson. Mater. Phys. Biol. Med. 4, 55–59 (1996)

    Article  Google Scholar 

  48. T.R. Hope, N.S. White, J. Kuperman, Y. Chao, G. Yamin, H. Bartch, N.M. Schenker-Ahmed, R. Rakow-Penner, R. Bussell, N. Nomura, S. Kesari, A. Bjørnerud, A.M. Dale, Demonstration of non-Gaussian restricted diffusion in tumor cells using diffusion time-dependent diffusion-weighted magnetic resonance imaging contrast. Front. Oncol. 6(3) (2016). https://doi.org/10.3389/fonc.2016.00179

  49. E. Sulyok, Z. Nyúl, P. Bogner, G. Sedin, Brain water and proton magnetic resonance relaxation in preterm and term rabbit pups: their relation to tissue hyaluronan. Neonatology 79, 67–72 (2001)

    Article  Google Scholar 

  50. J. Gustavsson, C. Cederberg, U.L.F. Sonesson, Global Food Losses and Food Waste-Extent, Causes and Prevention (Food Agriculture Organization of United Nations, Rome, 2011)

    Google Scholar 

  51. S. Grabowski, M. Marcotte, H.S. Ramaswamy, Drying of fruits, vegetables, and spices, in Handbook of Postharvest Technology: Cereals, Fruits, Vegetables, Tea, and Spices (Marcel Dekker, New York, 2003), pp. 653–95

    Google Scholar 

  52. M.I.H. Khan, R.M. Wellard, S.A. Nagy, M.U.H. Joardder, M.A. Karim, Investigation of bound and free water in plant-based food material using NMR T2 relaxometry. Innov. Food Sci. Emerg. Technol. 38(Part A), 252–261 (2016)

    Google Scholar 

  53. M.I.H. Khan, C. Kumar, M.U.H. Joardder, M.A. Karim, Determination of appropriate effective diffusivity for different food materials. Drying Technol. 35, 335–346 (2017)

    Article  Google Scholar 

  54. I.H. Khan, T. Farrell, S.A. Nagy, M.A. Karim, Fundamental understanding of cellular water transport process in bio-food material during drying. Sci. Rep. 8, Article number: 15191 (2018)

    Google Scholar 

  55. A.J. McElrone, B. Choat, G.A. Gambetta, C.R. Brodersen, Water uptake and transport in vascular plants. Nat. Educ. Knowl. 4, 6 (2013)

    Google Scholar 

  56. G.N. Agrios, Plant Pathology (Academic Press, New York, NY, 1997)

    Google Scholar 

  57. D.J. Beerling, P.J. Franks, Plant science: the hidden cost of transpiration. Nature 464, 495–496 (2010)

    Article  ADS  Google Scholar 

  58. M. Kramer, Osmosis is not driven by water dilution. Trends Plant Sci. 18, 195–197 (2013)

    Article  ADS  Google Scholar 

  59. L. Malińska, E. Rybska, E. Sobieszczuk-Nowicka, M. Adamiec, Teaching about water relations in plant cells: an uneasy struggle. CBE Life Sci. Educ. 15, ar78 (2016)

    Google Scholar 

  60. E. Steudle, Water transport across plant tissue: role of water channels. Biol. Cell 89, 259–273 (1997)

    Article  Google Scholar 

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Likhtenshtein, G.I. (2021). Hydration of Cells and Tissues. In: Biological Water. Soft and Biological Matter. Springer, Cham. https://doi.org/10.1007/978-3-030-82503-4_11

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