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Genetic Polymorphism β-Lactoglobulin Gene in Dubska Pramenka Sheep Breed

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CMBEBIH 2019 (CMBEBIH 2019)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 73))

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Abstract

Polymorphism exists in β-lactoglobulin (LGB) milk encoding gene in dairy animals, resulting in presence of different protein isoforms in milk. There is a link between genetic polymorphism in dairy proteins with technological properties of milk, milk yield and allergic reactions in humans. This study was conducted to identify LBG protein isoforms and their frequencies in population of Dubska Pramenka sheep breed. A total of 90 blood samples were genotyped using PCR-RFLP analysis. Genotypes were determined by PCR amplification followed by digestion with RsaI for identification of A and B alleles and using MspI for C allele identification. Two genetic variants (A and B) and three genotypes (AA, AB and BB) were identified. The presence of allele C in this study was not noticed. Gene frequencies of LGB A were 13; LGB B were 61; LGB AB were 61. There is no data on the genetic influence on the quality and quantity of milk from Dubska pramenka sheep, and there is need for further studies.

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Author information

Authors and Affiliations

  1. Inspekt-RGH d.o.o. Sarajevo, Hamdije Kreševljakovića 18/I, 71000, Sarajevo, Bosnia and Herzegovina

    Amela Masala

  2. Department of Chemistry, Biochemistry and Physiology, Faculty of Veterinary Medicine, University of Sarajevo, Zmaja od Bosne 90, 71000, Sarajevo, Bosnia and Herzegovina

    Ivona Alilović, Husein Ohran, Amina Hrkovic-Porobija & Aida Hodzic

  3. Animal Genetics Laboratory, University of Debrecen, Böszörményi str 138, 4032, Debrecen, Hungary

    Szilvia Kusza

  4. Department for Poultry Breeding, Production and Health Protection, Veterinary Faculty, University of Sarajevo, Zmaja od Bosne 90, 71000, Sarajevo, Bosnia and Herzegovina

    Teufik Goletic

Authors
  1. Amela Masala
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  2. Ivona Alilović
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  3. Husein Ohran
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  4. Szilvia Kusza
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  5. Teufik Goletic
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  6. Amina Hrkovic-Porobija
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  7. Aida Hodzic
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Correspondence to Ivona Alilović .

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Editors and Affiliations

  1. International Burch University, Sarajevo, Bosnia and Herzegovina

    Almir Badnjevic

  2. Faculty of Medicine, University of Banja Luka, Banja Luka, Bosnia and Herzegovina

    Ranko Škrbić

  3. International Burch University, Sarajevo, Bosnia and Herzegovina

    Lejla Gurbeta Pokvić

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Masala, A. et al. (2020). Genetic Polymorphism β-Lactoglobulin Gene in Dubska Pramenka Sheep Breed. In: Badnjevic, A., Škrbić, R., Gurbeta Pokvić, L. (eds) CMBEBIH 2019. CMBEBIH 2019. IFMBE Proceedings, vol 73. Springer, Cham. https://doi.org/10.1007/978-3-030-17971-7_97

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  • DOI: https://doi.org/10.1007/978-3-030-17971-7_97

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-17970-0

  • Online ISBN: 978-3-030-17971-7

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