Abstract
The aim of this pedagogical work is to enhance the understanding of fundamental recombinant protein production process and develop the required skills in pharmaceutical biotechnology of graduate students. In this study, the applied biotechnological module was reorganized by positioning specific scenarios of practical experiments in the central core of the course. We developed a training program by alternating lecture classes and practical lab experiments related to recombinant protein production process. Our results indicate that this new teaching method helps students in the understanding of different theoretical biotechnological concept and allows them to improve the abilities and skills required in recombinant protein production process.
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References
Expert Group on Future Skills Needs: Future Skills Requirements of the Biopharma-Pharmachem Sector (2010)
Elm’selmi, A., Boeuf, G., Elmarjou, A., Azouani, R.: Active pedagogy project to in-crease bio-industrial process skills. In: Auer, M., Guralnick, D., Uhomoibhi, J. (eds.) Interactive Collaborative Learning. ICL 2016. Advances in Intelligent Systems and Computing, vol. 544 (2016)
Gobert, J.D., Buckley, B.C.: Introduction to model-based teaching and learning in science education. Int. J. Sci. Educ. 22(9), 891–894 (2000)
Palomäki, E., Qvist, P., Natri, O., Joensuu, P., Närhi, M., Kähkönen, E., … Nordström, K.: LabLife3D: Teaching biotechnology and chemistry to engineering students by using Second Life. Stanford University, H-STAR Institute, USA; to Associate Professor Jukka M. Laitamäki, from New York University, USA, and to Professor Yngve Troye Nordkvelle from Lillehammer University, 124 (2011)
Miladi, B., Dridi, C., El Marjou, A., Boeuf, G., Bouallagui, H., Dufour, F., Di Martino, P., Elm’selmi, A.: An improved strategy for easy process monitoring and advanced purification of recombinant proteins. Mol. Biotechnol. 55(3), 227–235 (2013)
Rosano, G.L., Ceccarelli, E.A.: Recombinant protein expression in Escherichia coli: advances and challenges. Front. Microbiol. 5, 172 (2014)
Absi, R., Nalpace, C., Dufour, F., Huet, D., Bennacer, R., Absi, T.: Teaching fluid mechanics for undergraduate students in applied industrial biology: from theory to atypical experiments. Int. J. Eng. Educ. (IJEE) 27(3), 550–558 (2011)
Absi, R., Lavarde, M., Jeannin L.: Towards more efficiency in tutorials: active teaching with modular classroom furniture and movie-making project. In: 2018 IEEE Global Engineering Education Conference (EDUCON), pp. 774–778, 17–20 April 2018, Santa Cruz de Tenerife, Tenerife, Islas Canarias, Spain, Spain (2018)
Abbas, A.K., Lichtman, A.H., Pober, J.S.: Cellular and Molecular Immunology, 4th edn, pp. 235–269. W. B. Saunders Company, Philadelphia, PA (1991)
Horiuchi, T., Mitoma, H., Harashima, S.I., Tsukamoto, H., Shimoda, T.: Trans-membrane TNF-α: structure, function and interaction with anti-TNF agents. Rheumatology 49(7), 1215–1228 (2010)
Brustolim, D., Ribeiro-dos-Santos, R., Kast, R.E., Altschuler, E.L., Soares, M.B.P.: A new chapter opens in anti-inflammatory treatments: the antidepressant bupropion lowers production of tumor necrosis factor-alpha and interferon-gamma in mice. Int. Immunopharmacol. 6(6), 903–907 (2006)
Tobinick, E., Gross, H., Weinberger, A., Cohen, H.: TNF-alpha modulation for treatment of Alzheimer’s disease: a 6-month pilot study. Medscape Gen. Med. 8(2), 25 (2006)
Miladi, B., El Marjou, A., Boeuf, G., Bouallagui, H., Dufour, F., Di Martino, P., Elm’sel-mi, A.: Oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins. J. Biotechnol. 158(3), 97–103 (2012)
Peroutka III, R.J., Orcutt, S.J., Strickler, J.E., Butt, T.R.: SUMO fusion technology for enhanced protein expression and purification in prokaryotes and eukaryotes. In: Heterologous Gene Expression in E. coli, pp. 15–30. Humana Press (2011)
Crowe, J., Döbeli, H., Gentz, R., Hochuli, E., Stüber, D. & Henco, K.: 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. In: Harwood, A.J. (ed.) Methods in Molecular Biology, vol. 31, pp. 371–387. Humana Press, Inc., Totawa (1994)
Sherwood, R.: Protein fusions: bioseparations and application. Trends Biotechnol. 9, 1–3 (1991)
Schmidt, T.G.M., Skerra, A.: The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng. 6, 109–122 (1993)
Abel, U., Koch, C., Speitling, M., Hansske, F.G.: Modern methods to produce natural-product libraries. Curr. Opin. Chem. Biol. 6(4), 453–458 (2002)
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W., Prasher, D.C.: Green fluorescent protein as a marker for gene expression. Science 263, 802–805 (1994)
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Elm’selmi, A. et al. (2019). Enhancing Understanding and Skills in Biopharmaceutical Process Development: Recombinant Protein Production and Purification. In: Auer, M., Tsiatsos, T. (eds) The Challenges of the Digital Transformation in Education. ICL 2018. Advances in Intelligent Systems and Computing, vol 917. Springer, Cham. https://doi.org/10.1007/978-3-030-11935-5_37
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DOI: https://doi.org/10.1007/978-3-030-11935-5_37
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