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Genetic Engineering Solutions for Natural Products in Actinomycetes

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Handbook of Industrial Cell Culture

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Abstract

There are a number of genetic and molecular methods that are useful for the improvement of production of natural products in actinomycetes. These include traditional chemical mutagenesis and selection, transposition mutagenesis, targeted deletions and duplications by genetic engineering, and genetic recombination by protoplast fusion. With the recent developments in microbial genomics, transcriptome analysis, proteomics, metabolic reconstruction, and metabolite flux analysis, these new technologies are becoming valuable tools to aid in strain development. A robust strain development program can benefit from the synergy provided by coupling several of these approaches. This chapter limits its discussion to genetic engineering approaches to yield enhancement. More classical mutation and recombination approaches have been reviewed elsewhere (1–6).

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  1. Richard H. Baltz
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    Victor A. Vinci PhD

  2. Dow AgroSciences, Indianapolis, IN, USA

    Sarad R. Parekh PhD

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Baltz, R.H. (2003). Genetic Engineering Solutions for Natural Products in Actinomycetes. In: Vinci, V.A., Parekh, S.R. (eds) Handbook of Industrial Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-346-0_6

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