Abstract
Paramagnetic centers, such as naturally occurring free radicals and paramagnetic metal ions, are of crucial importance in many biological functions. Some examples are flavoenzymes, vitamin B12, aging, oxygen radicals, and melanins. While work in the early days of ESR was limited by the size and electronic constraints of the X-band cavity, as well as the need, frequently, to freeze-clamp the sample or excised tissue, an “ideal” in vivo ESR spectrometer is one in which a live animal can be directly accommodated (Swartz et al., 1972). These latter experiments were virtually impossible with commercial X-band (9.5 GHz) instruments as the large electric field losses through water and ions would result in “cooking the animal” besides the extreme difficulty in matching the sample. Conventional X-band EPR spectrometers present several technical limitations to measurements on live animals, principally microwave heating. Hence, the first attempts at EPR imaging were limited to very small nonliving samples.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Berliner, L. J., 1991, in EPR Imaging and In-Vivo EPR (Eaton, G., Eaton, S., and Ohno, K., eds.) CRC Press, Boca Raton, Fla., p. 291.
Berliner, L. J., and Fujii, H., 1985, Science 227: 517.
Berliner, L. J., and Fujii, H., 1986, J. Magn. Reson. 69: 68.
Berliner, L. J., and Wan, X., 1989, Magn. Reson. Med. 9: 430.
Berliner, L. J., Fujii, H., Wan, X., and Lukiewicz, S. J., 1987, Magn. Reson. Med. 4: 380
Brasch, R., 1983, Radiology 147: 781.
Eaton, G., Eaton, S., and Ohno, K., (eds.), 1991, EPR Imaging and In-Vivo EPR, CRC Press, Boca Raton, Fla.
Fujii, H., and Berliner, L. J., 1985, Magn. Reson. Med. 2: 275.
Fujii, H., and Berliner, L. J., 1986, J. Magn. Reson. 68: 377.
Koscielniak, J., and Berliner, L. J., 1991, in EPR Imaging and In-Vivo EPR (Eaton, G., Eaton, S., and Ohno, K., eds.), CRC Press, Boca Raton, Fla., p. 65.
Lukiewicz, S. J., and Lukiewicz, S. G., 1984, Magn. Reson. Med. 1: 297.
Maples, K. R., Jordan, S. J., and Mason, R. P., 1988, Mol. Pharmacol. 33: 344.
Mottley, C., and Mason, R. P., 1989, in Spin Labeling: Theory and Applications, Biological
Magnetic Resonance, Vol. 8 (Berliner, L. J., and Reuben, J., eds.), Plenum Press, New York, p. 489.
Nishikawa, H., Fujii, H., and Berliner, L. J., 1985, J. Magn. Reson. 62: 79.
Swartz, H., Bolton, J. R., and Borg, D. C. (eds.), 1972, Biological Applications of Electron Spin Resonance, Wiley, New York.
Swartz, H. M., Chen, K., Pals, M., Sentjurc, M. and Morse, P. D., 1986, Magn. Reson. Med. 3: 169.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media New York
About this chapter
Cite this chapter
Berliner, L.J., Fujii, H. (1992). Some Applications of ESR to in Vivo Animal Studies and EPR Imaging. In: Berliner, L.J., Reuben, J. (eds) In Vivo Spectroscopy. Biological Magnetic Resonance, vol 11. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9477-9_7
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9477-9_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9479-3
Online ISBN: 978-1-4757-9477-9
eBook Packages: Springer Book Archive