Abstract
Intrinsic properties of acidic sphingomyelinase cause major difficulties in attempts to produce a satisfactory antibody. The only two published papers do not carry conviction: the monoclonal antibody described by Freeman et al. (1983) was not demonstrated to precipitate sphingomyelinase activity while monospecificity of the polyclonal antibody prepared from a urine source by Weitz et al. (1985) is questionable.
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References
Freeman, S.J., Davidson, D.J., Shankaran, P. and Callahan, J.W., 1983, Monoclonal antibodies against human placental sphingomyelinase, Biosci. Rep., 3: 545–550
Holloway, P.W., 1973, A simple procedure for removal of Triton X-100 from protein samples, Anal.Biochem., 53: 304–308.
Jones, C.S., Shankaran, P., Davidson, D.J., Poulos, A. and Callahan, J.W., 1983, Studies on the structure of sphingomyelinase, Biochem.J., 209: 291–297.
Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature (London) 227: 680–685.
Rousson, R., Vanier, M.T. and Louisot, P., 1983, Chromatofocusing of purified placental sphingomyelinase, Biochimie, 65: 115–120.
Towbin, H., Staehelin, T. and Gordon, J., 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc.Natl.Acad.Sci. USA, 76: 4350–4354.
Vanier, M.T., Revol, A. and Fichet, M., 1980, Sphingomyelinase activities of various human tissues in control subjects and in Niemann-Pick disease. Development and evaluation of a microprocedure, Clin.Chim. Acta, 106: 257–267.
Vanier, M.T., Rousson, R. and Louisot, P., 1983, Chromatofocusing of skin fibroblast sphingomyelinase: alterations in Niemann-Pick disease C shared by GM1-gangliosidosis, Clin.Chim.Acta, 130: 155–161.
Weitz, G., Driessen, M., Brouwer-Kelder, E.M., Sandhoff, K., Barranger, J.A., Tager, J.M. and Schram, A.W., 1985, Soluble sphingomyelinase from human urine as antigen for obtaining anti-sphingomyelinase antibodies, Biochim.Biophys.Acta, 838: 92–97.
Yamanaka, T. and Suzuki, K., 1982, Acid sphingomyelinase of human brain: purification to homogeneity, J.Neurochem., 38: 1753–1764.
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© 1986 Plenum Press, New York
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Rousson, R., Vanier, M.T., Louisot, P. (1986). Immunological Studies on Acidic Sphingomyelinase. In: Freysz, L., Dreyfus, H., Massarelli, R., Gatt, S. (eds) Enzymes of Lipid Metabolism II. NATO ASI Series, vol 116. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5212-9_38
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DOI: https://doi.org/10.1007/978-1-4684-5212-9_38
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