Abstract
Cyanobactrial blooms are common throughout the world in eutrophic water bodies. Cyanobacterial hepato- and neurotoxins represent a health threat for humans as well as for animals in drinking water supplies. Mouse bioassays have been used extensively to detect cyanobacterial blooms toxicity but the use of warm-blooded vertebrates is limited to specialised laboratories and poses ethical problems. Hence, it is necessary to find alternative assays to detect and quantify cyanobacterial hazards. This study reports on the toxicity of 7 cyanobacterial bloom samples from the Jeziorsko. Reservoir in central Poland as determined with 4 microbiotests: the Thamnotoxkit F™, the Daphtoxkit F™ magna, the ProtoxkitF™ and the Spirotox. The phytoplankton samples were lyophilized, sonicated and passed through octadecyl cartridges. The results were compared to those obtained with mouse bioassays and HPLC analysis of microcystin-LR (MC/LR) in the bloom samples..
24h LC50s for the Thamnotoxkit F™ and the Spirotox tests showed statistically significant correlations with MC/LR concentrations in the samples. In turn, no statistically significant correlations were found between mouse 24h LD50s and microbiotest 24h L(E)C50s which indicates that cyanobacterial hepatotoxins may exert different effects in microbiotests with invertebrates and in mouse bioassays.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Algaltoxkit F™. 1996. Freshwater Toxicity Test with Microalgae. Standard Operatonal Procedure. Creasel, Deinze, Belgium. 28 pages.
Artoxkit F™. 1990. Artemia Toxicity Screening Test for Estuarine and Marine Waters. Standard Operational Procedure. Creasel, Deinze, Belgium. 22 pages.
Daphtoxkit F™ magna. 1996. Crustacean Toxicity Screening Test for Freshwater. Standard Operational Procedure. Creasel, Deinze, Belgium. 16 pages.
Microbics Corporation, 1992 Microtox® Manual, Volsl-5. Carlsbad, CA 2008-8883, USA
Di Toro D.M. 1990. A review of the data supporting the equilibrium partitioning approach to establish sediment quality criteria. In: Proceedings, Symposium/ Workshop on Contaminated Marine Sediments, Tampa, Florida. Marine Board, National Research Council, Washington, DC.
Nalecz-Jawecki G. and Sawicki J. 1998. Toxicity of inorganic compounds in Spirotox test-a miniaturised version of the Spirostomum ambiguum test. Arch Environ Contam Toxicol 34:1–5.
OECD 1984. Guidelines for the Testing of Chemicals. Guideline 201. Alga, Growth Inhibition Test, pp 1–14.
OECD 1986. Guidelines for the Testing of Chemicals. Guideline 202. Daphnia sp. Acute Immobilisation and Reproduction Test, 1–16.
Rotoxkit F™. 1992. Rotifer Toxicity Screening Test for Freshwater. Standard Operational Procedure. Creasel, Deinze, Belgium. 22 pages.
Rotoxkit M™. 1994. Rotifer Toxicity Screening Test for Freshwater. Standard Operational Procedure. Creasel, Deinze, Belgium. 23 pages.
Thamnotoxkit F™. 1995. Crustacean Toxicity Screening Test for Freshwater. Standard Operational Procedure. Creasel, Deinze, Belgium. 23 pages.
Vangheluwe M., Janssen C.R. and Persoone G. 1996. Ecotoxicological evaluation of sediments in Flanders: evaluation and selection of ecotoxicity tests (in Dutch). Water, 89,173–177.
Lahti K., Ahtianen J., Rapala J., Sivonen K. and Niemela S.I. 1995. Assessment of rapid bioassays for detecting cyanobacterial toxicity. Lett. Appl. Microbiol. 21: 109–114.
Lawton L.A., Beattie K.A., Hawser S.P., Campbell D.L. and Codd G.A. 1994. Evaluation of assay methods for the determination of cyanobacterial hepatotoxicity. In Codd G.A. and Jefferies T.M., eds, Detection Methods for Cyanobacterial Toxins. The Royal Society of Chemistry, Cambridge, UK, pp 111–116.
Nalecz-Jawecki G. and Sawicki J. 1998. Toxicity of Inorganic Compounds in the Spirotox Test: A Miniaturized Version of the Spirostomum ambiguum Test. Arch.Environ.Contam.Toxicol. 34: 1–5.
Protoxkit F™ 1998. Freshwater toxicity test with a ciliate protozoan. Standard Operational Procedure. Creasel, Deinze, Belgium. 18 pages.
Reinikainen M., Kiviranta J., Ulvi V. and Niku-Paavola M.-L. 1995. Acute toxic effects of a novel cyanobacterial toxin on the crustaceans Artemia salina and Daphnia pulex. Arch.Hydrobiol. 133: 61–69.
Sivonen K., Niemela S.I., Niemi R.M., Lepisto L., Luoma T.H. and Rasanen L.A. 1990. Toxic cyanobacteria (blue-green algae) in Finnish fresh and coastal waters. Hydrobiologia 190: 267–275.
Thamnotoxkit F™ 1995. Crustacean toxicity screening test for freshwater. Standard Operational Procedure. Creasel, Deinze, Belgium. 23 pages.
Weber C.I. 1991. Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms. EPA-600/4-90-027. Acute toxicity data analysis. Environmental Protection Agency. Cincinnati, Ohio, USA. pp 75–119.
Yasuno M. and Sugaya Y. 1991. Toxicities of Microcystis viridis and isolated hepatotoxic polypeptides on cladocerans. Verh.Int.Verein.Limnol. 24: 2622.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media New York
About this chapter
Cite this chapter
Tarczynska, M., Nalecz-Jawecki, G., Brzychcy, M., Zalewski, M., Sawicki, J. (2000). The toxicity of cyanobacterial blooms as determined by microbiotests and mouse assays. In: Persoone, G., Janssen, C., De Coen, W. (eds) New Microbiotests for Routine Toxicity Screening and Biomonitoring. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4289-6_59
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4289-6_59
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6924-0
Online ISBN: 978-1-4615-4289-6
eBook Packages: Springer Book Archive