Abstract
The application of immunochemical methods in veterinary drug residues is discussed. These methods concern (i) immunochemical determinations such as ELISAs for rapid screening purposes and (ii) immunoaffinity clean-up techniques for crude samples or sample extracts prior to a physicochemical determination. Examples of these methods are given. Special attention is paid to the detection and/or determination of chloramphenicol residues by these techniques. This concerns a rapid detection method using a streptavidin-biotin ELISA and an immunoaffinity clean-up, both off-line and by means of a fully automated HPLC system.
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Haagsma, N., 1990, Sample preparation in drug residue analysis, in: “Proceedings of the EuroResidue Conference”, Noordwijkerhout, The Netherlands, May 1990, N. Haagsma, A. Ruiter and P.B. Czedik-Eysenberg, eds., State University, Utrecht, p. 40.
Smith, C.J., 1990, Evolution of the immunoassay. In: Development and application of immunoassay for food analysis. J.H. Rittenberg Ed., Elsevier Applied Science, London/New York, p. 3.
Köhler, G., and Milstein, C., 1975, Continuous cultures of fused cells secreting antibodies of predefined specificity, Nature 256: 495.
Yalow, R.S., and Berson, S.A., 1959, Assay of plasma insulin in human subjects by immunological methods, Nature 184: 1648.
Ekins, R.P, 1960, The estimation of thyroxine in human plasma by an electrophoretic technique, Clin. Chim. Acta 5:453.
Engvall, E., and Perlmann, P., 1971, Enzyme-linked immunosorbent assay (ELISA), Quantitative assay of immunoglobulin G, Immunochemistry 8: 871.
Weemen, B.K. van, and Schuurs, A.H.W.M., 1971, Immunoassay using antigen-enzymeconjugates, FEBS Letters 15: 232.
Morris, B.A., and Clifford, M.N., 1985, “Immunoassays in food analysis”, Elsevier Applied Science publishers, London.
Benraad, Th.J., Stephany, R.W., Rosmalen, F.M.A., Hofman, J.A., Loeber, J.G., and Evers, L.H., 1981, The necessity of chromatographic purification prior to radio-immunoassay of diethylstilbestrol in the urine of cattle. Vet. O., 3:153.
Rattenberger, E., Matzke, P., and Neudegger, J., 1985, Entwicklung eines Radioimmunotests zur Erfassung von Rückständen des ß-Rezeptorenblockers Carazolol in Blut und Ham von Schweinen, Arch. Lebensmit-telhyg., 36:85.
Arnold, D., Berg, D. vom, Boertz, A.K., Mallick, U., and Somogyi, A., 1984, Radioimmunologische Bestimmung von Chloramphenicol-Rückständen in Muskulatur, Milch und Eier, Arch. Lebensmittelhyg., 35:131.
Porstmann, T., Porstmann, B., and Seifert, R., 1983, Application of the peroxidase-antiperoxidase system as an universal reagent for the two-site binding enzyme immunoassay, Clin. Chim. Acta, 129:107.
Stanley, C.J., Johannsson, A., and Self, C.H., 1985, Enzyme amplification can enhance both the speed and the sensitivity of immunoassays, J. Immunol. Methods, 83:89.
Carr, R.I., Mansour, M., Sadi, D., James, H., and Jones, J.V., 1987, A substrate amplification system for enzyme-linked immunoassays. Demontration of its general applicability to ELISA systems for detecting antibodies and immune complexes, J. Immunol. Methods, 98:201.
Wilchek, M., and Bayer, E.A., 1988, The avidin-biotin complex in bioanalytical applications, Anal. Biochem., 171:1.
Guesdon, J., Ternynck, T., and Avrameas, S., 1979, The use of avidin-biotin interaction in immunoenzymatic techniques, J. Histochem. Cytochem., 27:1131.
Fleeker, J.R., and Lovett, L.J., 1985, Enzyme immunoassay for screening sulfamethazine in swine blood, J. Assoc. Off. Anal. Chem., 68:172.
Singh, P., Ram, B.P., and Sharkvo, N., 1989, Enzyme immunoassay for screening of sulfamethazine in swine, J. Agric Food Chem., 37:109.
Dixon-Holland, D.E., and Katz, S.E., 1988, Competitive direct enzyme-linked immunosorbent assay for detection of sulfamethazine residues in swine urine and muscle tissue, J. Assoc. Off. Anal. Chem., 71:1137.
Dixon-Holland, D.E., and Katz, S.E., 1988, Direct competitive enzyme-linked immunosorbent assay for sulfamethazine residues in milk, J. Assoc. Off. Anal. Chem., 72:447.
Zwickl, C.M., Smith, H.W., and Bick, P.H., 1990, Rapid and sensitive ELISA method for the determination of bovine somatotropin in blood and milk, J. Agric. Food Chem., 38:1358.
Kitagawa, T., Gotoh, Y., Uchiyama, K., Kohri, Y., Kinoue, T., Fujiwara, K., and Ohtani, W., 1988, Sensitive immunoassay of cephalexin residues in milk, hen tissues and eggs, J. Assoc. Off. Anal. Chem., 71:915.
Märtlbauer, E. and Terplan, G., 1987, Ein enzymimmunologischer Nachweis von Chloramphenicol in Milch, Arch. Lebensmittelhyg., 38:3.
Water, C. van de, and Haagsma, N., 1990, A sensitive streptavidin-biotin enzyme-linked immunosorbent assay for rapid screening of residues of chloramphenicol in milk, Food Agr, Immunol., 2:11.
Water, C. van de, and Haagsma, N., 1991, Analysis of chloramphenicol residues in swine tissues and milk: comparative study using different screening and quantitative methods, J.Chromatogr., 566:173.
Water, C. van de, Haagsma, N., Kooten, P.J.S. van, and Eden, W. van, 1987, An enzyme-linked immunosorbent assay for the determination of chloramphenicol using a monoclonal antibody, Z. Lebensm. Unters. Forsch., 185:202.
Water, C. van de, and Haagsma, N., 1990, A sensitive streptavidin-biotin enzyme-linked immunosoebent assay for rapid screening of residues of chloramphenicol in swine muscle tissues, J. Assoc. Off. Anal. Chem., 73:534.
Mount, M.E., and Failla, D.L., 1987, Production of antibodies and development of enzyme immunoassays for determination of monensin in biological samples, J. Assoc. Off, Anal. Chem., 70:201.
Nouws, J.F.M., Beek, F., Aerts, M.M.L., Baakman, M., and Laurensen, J., 1987, Monitoring slaughtered animals for chloramphenicol residues by an immunoassay test kit (Quik-card), Arch. Lebensmittelhyg., 38:9.
Nouws, J.F.M., Laurensen, J., and Aerts, M.M.L., 1987, Monitoring of chloramphenicol residues in eggs by HPLC and an immunoassay (QuikCard), Arch. Lebensmittelhyg., 38:7.
Nouws, J.F.M., Laurensen, J., and Aerts, M.M.L., 1988, Monitoring milk for chloramphenicol residues by an immunoassay (Quik-card), Vet. O., 10:270.
Haagsma, N., Schreuder, C., and Rensen, E.R.A., 1986. Rapid sample preparation method for the determination of chloramphenicol in swine muscle by high-performance liquid chromatography, J. Chromatogr., 363:353.
Cuatrecasas, P., Wilchek, M., and Anfinsen, C.B., 1968, Selective enzyme purification by affinity chromatography, Proc. Natl. Acad. Sci. U.S.A., 61:636.
Livingstone, D.M., 1974, Immunoaffinity chromatography of proteins, in: “Methods in Enzymology”, vol. XXIV, W.B. Jakoby and M. Wilchek, eds., p. 723.
Katz, S.E., and Brady, M.S., 1990, High-performance immunoaffinity chromatography for drug residue analysis, J. Assoc. Off. Anal. Chem., 73:557.
Ginkel, L.A. van, 1991, Immunoaffinity chromatography, its applicability and limitations in multi-residue analysis of anabolizing and do** agents, J. Chromatogr., 564:363.
Dean, P.D.G., Johnson, W.S., and Middle, F.A., eds., 1985, “Affinity chromatography, a practical approach”, IRL Press Ltd., Oxford.
Bethell, G.S., Ayers, J.S., Hearn, M.T.W., and Hancock, W.S., 1981, Investigation of the activation of various insoluble polysaccharides with 1,1’carbonyldiimidazole and of the properties of the activated matrices, J. Chromatogr., 219:361.
Hearn, M.T.W., Harris, E.L., Bethell, G.S., Hancock, W.S., and Ayers, J.A., 1981, Application of 1,1’-carbonyldiimidazole-activated matrices for the purification of proteins. III. The use of 1,1’-carbonyldiimidazole-activated agaroses in the biospecific affinity chromatographic isolation of serum antibodies, J. Chromatogr., 218:509.
Prisyazhnoy, V.S., Fusek, M., and Alakhov, Y.B., 1988, Synthesis of high-capacity immunoaffinity sorbents with oriented immobilized immunoglobulins or their Fab’ fragments for isolation of proteins, J. Chromatogr. Biomed. Appl., 424:243.
Matson, R.S., and Little, M.C., 1988, Strategy for the immobilization of monoclonal antibodies on solid-phase supports, J. Chromatogr., 458:67.
Scatchard, G., 1949, The attractions of proteins for small molecules and ions, Ann. N.Y. Acad. Sci., 51:660.
Oss, C.J. van, Good, R.J., and Chaudhury, M.K., 1986, Nature of the antigen-antibody interaction. Primary and secondary bonds: optimal conditions for association and dissociation, J. Chromatogr. Biomed. Appl., 376:111.
Ginkel, L.A. van, Blitterswijk, H. van, Zoontjes, P.W., Bosch, D. van den, and Stephany, R.W., 1988, Assay for trenbolone and its metabolite 17atrenbolone in bovine urine based on immunoaffinity chromatographic clean-up and off-line high-performance liquid chromatography - thin-layer chromatography, J. Chromatogr., 445:385.
Ginkel, L.A. van, Stephany, R.W., Rossum, H.J. van, Blitterswijk, H. van, Zoontjes, P.W., Hooyschuur, R.C.M., and Zuyderdorp, J., 1989, Effective monitoring of residues of nortestosterone and its major metabolite in bovine urine and bile, J. Chromatogr., 489:95.
Ginkel, L.A. van, Stephany, R.W., Rossum, H.J. van, Steinbuch, H.M., Zomer, G., Heeft, E. van de, and Jong, A.P.J.M. de, 1989, Multi-immunoaffinity chromatography: a simple and highly selective clean-up method for multi-anabolic residue analysis of meat, J. Chromatogr., 489:111.
Farjam, A., Jong, G.J. de, Frei, R.W., Brinkman, U.A. Th., Haasnoot, W., Hamers, A.R.M., Schilt, R. and Huf, F.A., 1988, Immunoaffinity pre-column for selective on-line sample pretreatment in high-performance liquid chromatography determination of 19-testosterone, J. Chromatogr., 452:419.
Haasnoot, W., Schilt, R., Hamers, A.R.M., Huf, F.A., Farjam, A., Frei, R.W., and Brinkman, U.A.Th., 1989, Determination of ß-19-nortestosterone and its metabolite α-19-nortestosterone in biological samples at the subparts per billion level by high-performance chromatography with online immunoaffinity sample pretreatment, J. Chromatogr., 489:157.
Bagnati, R., Castelli, M.G., Airoldi, L., Oriundi, M.P., Ubaldi, A., and Fanelli, R, 1990, Analysis of diethylstilbestrol, dienestrol and hexestrol in biological samples by immunoaffinity extraction and gas chromatography - negative-ion chemical ionization mass spectrometry, J. Chromatogr., 527:267.
Bagnati, R., and Fanelli, R., 1991, Determination of 19-nortestosterone, testosterone and trenbolone by gas chromatograpy - negative-ion mass spectrometry after formation of the pentafluorobenzylcarboxymethoximetrimethylsilyl derivatives, J. Chromatogr., 547:325.
Schilt, R., Haasnoot, W., Jonker, M.A., Hooyerink, H., and Paulussen, R.J.A., 1990, Determination of ß-agonistic drugs in feed, urine and tissue samples of cattle with immunoaffinity clean-up and GC-MS, in: “Proceedings of the EuroResidue Conference”, Noordwijkerhout, The Netherlands, May 1990, N. Haagsma, A. Ruiter and P.B. Czedik-Eysenberg, eds., State University, Utrecht, p. 320.
Ginkel, L.A. van, Stephany, R.W., Rossum, H.J. van, and Fula, J., 1991, Multiresidue test for ß-agonists in a variety of matrices, in: “Proceedings of the International Symposium on Veal Calf Production”, Wageningen, The Netherlands, March 1990, J.H.M. Metz and C.M. Groenestein, eds., Pudoc, Wageningen, 192.
Haasnoot, W., Ploum, M.E., Paulussen, R.J.A., Schilt, R., and Huf, F., 1990, Rapid determination of clenbuterol residues in urine by high-performance liquid chromatography with on-line automated sample processing using immunoaffinity chromatography, J. Chromatogr., 519:323.
Water, C. van de, and Haagsma, N., 1987, Determination of chloramphenicol in swine muscle tissue using a monoclonal antibody-mediated clean-up procedure, J. Chromatogr., 411:415.
Water, C. van de, Tebbal, D., and Haagsma, N., 1989, Monoclonal antibody-mediated clean-up procedure for the high-performance liquid chromatographic analysis of chloramphenicol in milk and eggs, J. Chromatogr., 478:205.
Moretti, V.M., Water, C. van de, and Haagsma, N., 1991, Automated determination of chloramphenicol in milk and swine muscle tissue using on-line immunoaffinity sample clean-up and column switching technique, in preparation.
Clovis, Y.D., 1987, Matrix evaluation for preparative high-performance affinity chromatography, J. Chromatogr., 407:179.
Josic, D., Hofmann, W., Habermann, R., Becker, A., and Reutter, W., 1987, High-performance liquid affinity chromatography of liver plasma membrane proteins, J. Chromatogr., 397:39.
Phillips„ T.M., and Frantz, S.C., 1988, Isolation of specific lymphocyte receptors by high-performance immunoaffinity chromatography, J. Chromatogr., 444:13.
Nilsson, B., 1983, Extraction and quantitation of cortisol by use of high-performance liquid chromatography, J. Chromatog. Biomed. Appl., 276:413.
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Haagsma, N., van de Water, C. (1992). Immunochemical Methods in the Analysis of Veterinary Drug Residues. In: Agarwal, V.K. (eds) Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3356-6_7
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DOI: https://doi.org/10.1007/978-1-4615-3356-6_7
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