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Assembly of Large Icosahedral Double-Stranded RNA Viruses

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Viral Molecular Machines

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 726))

Abstract

Double-stranded RNA (dsRNA) viruses are a diverse group of viruses infecting hosts from bacteria to higher eukaryotes. Among the hosts are humans, domestic animals, and economically important plant species. Fine details of high-resolution virion structures have revealed common structural characteristics unique to these viruses including an internal icosahedral capsid built from 60 asymmetric dimers (120 monomers!) of the major coat protein. Here we focus mainly on the structures and assembly principles of large icosahedral dsRNA viruses belonging to the families of Cystoviridae and Reoviridae. It is obvious that there are a variety of assembly pathways utilized by different viruses starting from similar building blocks and reaching in all cases a similar capsid architecture. This is true even with closely related viruses indicating that the assembly pathway per se is not an indicator of relatedness and is achieved with minor changes in the interacting components.

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Abbreviations

dsRNA:

Double-stranded RNA

mRNA:

Messenger RNA

ssRNA:

Single-stranded RNA

Cryo-EM:

Cryo-electron microscopy

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Acknowledgments

This work was supported by the Finnish Center of Excellence Program (2006–2011) of the Academy of Finland (grant 1129684) and Academy of Finland grants 250113 and 256069.

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  1. Department of Biosciences and Institute of Biotechnology, University of Helsinki, Viikki Biocenter, 00014, Helsinki, Finland

    Minna M. Poranen & Dennis H. Bamford

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  1. Minna M. Poranen
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  2. Dennis H. Bamford
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Correspondence to Minna M. Poranen .

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  1. Dept. Biological Sciences, Purdue University, W. State St. 915, West Lafayette, 47907-2054, Indiana, USA

    Michael G. Rossmann

  2. Dept. Biology, Catholic University of America, Washington, 20064, District of Columbia, USA

    Venigalla B. Rao

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Poranen, M.M., Bamford, D.H. (2012). Assembly of Large Icosahedral Double-Stranded RNA Viruses. In: Rossmann, M., Rao, V. (eds) Viral Molecular Machines. Advances in Experimental Medicine and Biology, vol 726. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-0980-9_17

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