Summary
Whole-cell currents of type I cells were characterized using the patch-clamp technique. At least four types of currents could be separated: 1) outward rectifying currents, which were sensitive to Cs+ and tetraethylammonium (TEA); 2) Ca2+-dependent outward currents (Co2+ sensitive); 3) TT**nsensitive slowly inactivating inward currents blocked by organic and inorganic Ca2+-channel blockers; 4) Na+ currents were observed also but not analyzed. Decreasing Po2 in the bath did not affect the Ca2+-inward currents but reduced the amplitude of the outward rectifying K+ current.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Eyzaguirre C, Fidone SJ (1980). Transduction mechanisms in carotid body: glomus cells, putative neurotransmitters, and nerve endings. Am J Physiol 239: C135 - C152.
Fidone S, Gonzalez C, Yoshizaki K (1982). Effects of low oxygen on the release of dopamine from the rabbit carotid body in vitro. J Physiol (Lond) 333: 93–110.
Delpiano MA, Acker H (1989). Hypoxic and hypercapnic responses of [Ca2+]o and [K+]o in the cat carotid body in vitro. Brain Res 482: 235–246.
Hamill OP, Marty A, Neher E, Sakmann E, Sigworth FJ (1981). Improved patch-clamp techniques for high-resolution current recordings from cells and cell-free membrane patches. Pflügers Arch 391: 85–100.
Pietruschka F (1974). Cytochemical demonstration of catecholamines in cells of the carotid body in primary tissue culture. Cell Tiss Res 151: 317–321.
Connor JA, Stevens CF (1971). Voltage clamp studies of a transient outward membrane current in gastropod neural somata. J Physiol (Lond) 213: 21–30.
Hescheler J, Delpiano MA, Acker H, Pietruschka F (1989). Ionic currents on type-I cells of the rabbit carotid body measured by voltage-clamp experiments and the effect of hypoxia. Brain Res 486: 79–88.
Pietruschka F (1985). Calcium influx in cultured carotid body cells is stimulated by acetylcholine and hypoxia. Brain Res 347: 140–143.
Belles B, Malécot CO, Hescheler J, Trautwein W (1988). ‘Rundown’ of the Ca current during long whole-cell recordings in guinea pig heart cells: role of phosphorylation and intracellular calcium. Pflügers Arch 411:353–360.
Obeso A, Almaraz L, Gonzalez C (1989). Effects of cyanide and uncouplers on chemoreceptor activity and ATP content of the cat carotid body. Brain Res 481: 250–257.
Delpiano MA, Hescheler J (1989). Evidence for a Po2 sensitive K+ channel in the type-I cell of the rabbit carotid body. FEBS Letters 249: 195–198.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag New York Inc.
About this paper
Cite this paper
Hescheler, J., Delpiano, M.A. (1990). Ionic Currents in Carotid Body Type I Cells and the Effects of Hypoxia and NaCN. In: Eyzaguirre, C., Fidone, S.J., Fitzgerald, R.S., Lahiri, S., McDonald, D.M. (eds) Arterial Chemoreception. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3388-6_8
Download citation
DOI: https://doi.org/10.1007/978-1-4612-3388-6_8
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-7993-8
Online ISBN: 978-1-4612-3388-6
eBook Packages: Springer Book Archive