Abstract
Root metaproteome analysis can reveal the functions that govern plant-microbe and microbe-microbe interactions under specific environmental conditions. Efficient protein extraction method from microbes associated with plant roots is crucial for the comprehensive analysis of the metaproteome. In this chapter, a straightforward protein extraction method for roots of Arabidopsis inoculated with a microbial community that uses only milligrams of tissue is outlined. In addition, the plant inoculation using a synthetic community (SynCom) and the methods for a nanoflow liquid chromatography coupled to a high-resolution/high-accuracy mass spectrometer (LC-MS/MS) are described.
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Salvato, F., Kleiner, M. (2024). A Complete Metaproteomic Workflow for Arabidopsis Roots Inoculated by Synthetic Bacteria. In: Salerno, C. (eds) Metaproteomics. Methods in Molecular Biology, vol 2820. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3910-8_7
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DOI: https://doi.org/10.1007/978-1-0716-3910-8_7
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