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Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling

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Lipid Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2816))

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Abstract

Intracellular Ca2+ can be conveniently monitored by sensitive Ca2+ fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca2+ imaging. Here we describe the protocols to measure intracellular Ca2+ for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.

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Acknowledgments

This work was partially supported by National Institutes of Health (NIH) Grants S10 OD025230 and R15 HL168628 (to ZP). J.Y.Y was supported by NIH R25 HL163861 through UTA SURPINT Research Program Fellowship.

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Authors and Affiliations

  1. Department of Graduate Nursing, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA

    Yan Chang, Marco Brotto & Zui Pan

  2. Bone and Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA

    Yan Chang, Marco Brotto & Zui Pan

  3. Department of Kinesiology, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA

    James Y. Yi & Zui Pan

Authors
  1. Yan Chang
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  2. James Y. Yi
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  3. Marco Brotto
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  4. Zui Pan
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Corresponding author

Correspondence to Zui Pan .

Editor information

Editors and Affiliations

  1. Bone-Muscle Research Center, University of Texas at Arlington, Arlington, TX, USA

    Marco Brotto

  2. Bascom Palmer Eye Institute, University of Miami, Miami, FL, USA

    Sanjoy K. Bhattacharya

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Chang, Y., Yi, J.Y., Brotto, M., Pan, Z. (2024). Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling. In: Brotto, M., Bhattacharya, S.K. (eds) Lipid Signalling. Methods in Molecular Biology, vol 2816. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3902-3_7

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  • DOI: https://doi.org/10.1007/978-1-0716-3902-3_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3901-6

  • Online ISBN: 978-1-0716-3902-3

  • eBook Packages: Springer Protocols

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