Chlorophyll Fluorescence on the Fast Timescale

  • Protocol
  • First Online:
Photosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2790))

  • 326 Accesses

Abstract

Chlorophyll fluorescence is a rapid and noninvasive tool used for probing the activity of photosynthesis that can be used in vivo and in the field. It is highly relevant to the demands of high-throughput crop phenoty** and can be automated or manually applied. In this chapter, we describe protocols and advice for making fast timescale fluorescence measurements using handheld equipment in the laboratory or in the field in the context of phenoty**. While interpretation of some measured parameters requires caution for the purpose of identifying underlying mechanisms, we demonstrate this technique is appropriate for some applications where convenience, rapidity, and sensitivity are required.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
GBP 34.95
Price includes VAT (United Kingdom)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
GBP 159.50
Price includes VAT (United Kingdom)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
GBP 199.99
Price includes VAT (United Kingdom)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Pask AJD, Pietragalla J, Mullan D, Reynolds MP (eds) (2012) Physiological breeding II: a field guide to wheat phenoty**. International Wheat and Maize Improvement Center (CIMMYT), Mexico

    Google Scholar 

  2. Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulated fluorometer. Photosynth Res 10:51–62

    Article  CAS  PubMed  Google Scholar 

  3. Strasser RJ, Govindjee (1991) The F0 and the O–J–I–P fluorescence rise in higher plants and algae. In: Argyroudi-Akoyunoglou JH (ed) Regulation of chloroplast biogenesis. Plenum Press, New York, pp 423–426

    Google Scholar 

  4. Murchie EH, Lawson T (2013) Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. J Exp Bot 64:3983–3998

    Article  CAS  PubMed  Google Scholar 

  5. Keller B, Matsubara S, Rascher U, Pieruschka R, Steier A, Kraska T, Muller O (2019) Genotype specific photosynthesis x environment interactions captured by automated fluorescence canopy scans over two fluctuating growing seasons. Front Plant Sci 10:481568. https://doi.org/10.3389/FPLS.2019.01482/BIBTEX

    Article  Google Scholar 

  6. Kimm H, Guan K, Burroughs CH, Peng B, Ainsworth EA, Bernacchi CJ, Moore CE, Kumagai E, Yang X, Berry JA, Wu G (2021) Quantifying high-temperature stress on soybean canopy photosynthesis: the unique role of sun-induced chlorophyll fluorescence. Glob Chang Biol 27(11):2403–2415. https://doi.org/10.1111/GCB.15603

    Article  CAS  PubMed  Google Scholar 

  7. Lazár D (2006) The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light. Funct Plant Biol 33:9–30

    Article  PubMed  Google Scholar 

  8. Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Kluwer Academic Publishers, Dordrecht, pp 321–362

    Chapter  Google Scholar 

  9. Stirbet A, Govindjee. (2011) On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: basics and applications of the OJIP fluorescence transient. J Photochem Photobiol B 104:236–257. Strasser RJ, Srivastava A, Govindjee (1995) Polyphasic chlorophyll a fluorescence transients in plants and cyanobacteria. Photochem Photobiol 61:32–42

    Google Scholar 

  10. Ajigboye OO, Murchie EH, Ray RV (2014) Foliar application of isopyrazam and epoxiconazole improves photosystem II efficiency, biomass and yield in winter wheat. Pest Biochem Physiol 114:52–60

    Article  CAS  Google Scholar 

  11. Ajigboye OO, Bousquet L, Murchie EH, Ray RV (2016) Chlorophyll fluorescence parameters allow the rapid detection and differentiation of plant responses in three different wheat pathosystems. Funct Plant Biol 43(4):356–369

    Article  CAS  PubMed  Google Scholar 

  12. Spyroglou I, Rybka K, Maldonado Rodriguez R, Stefański P, Valasevich NM (2021) Quantitative estimation of water status in field-grown wheat using beta mixed regression modelling based on fast chlorophyll fluorescence transients: a method for drought tolerance estimation. J Agron Crop Sci 207(4):589–605. https://doi.org/10.1111/JAC.12473

    Article  CAS  Google Scholar 

  13. da Silva JM, Figueiredo A, Cunha J, Eiras-Dias JE, Silva S, Vanneschi L, Mariano P (2020) Using rapid chlorophyll fluorescence transients to classify Vitis genotypes. Plants 9(2):174. https://doi.org/10.3390/PLANTS9020174

    Article  PubMed  PubMed Central  Google Scholar 

  14. Ripoll J, Bertin N, Bidel LPR, Urban L (2016) A user’s view of the parameters derived from the induction curves of maximal chlorophyll a fluorescence: perspectives for analysing stress. Front Plant Sci 7:1679

    Article  PubMed  PubMed Central  Google Scholar 

  15. Ajigboye OO, Lu C, Murchie EH, Schlatter C, Swart G, Ray RV (2017) Altered gene expression by sedaxane increases PSII efficiency, photosynthesis and growth and improves tolerance to drought in wheat seedlings. Pest Biochem Physiol 137:49–61

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Erik H. Murchie .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Ajigboye, O.O., Ray, R.V., Murchie, E.H. (2024). Chlorophyll Fluorescence on the Fast Timescale. In: Covshoff, S. (eds) Photosynthesis . Methods in Molecular Biology, vol 2790. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3790-6_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-3790-6_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3789-0

  • Online ISBN: 978-1-0716-3790-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Navigation