Abstract
It is critical that human adipose-derived stromal/stem cell (hASC) tissue engineering therapies possess appropriate mechanical properties in order to restore the function of the load-bearing tissues of the musculoskeletal system. In an effort to elucidate hASC response to mechanical stimulation and develop mechanically robust tissue-engineered constructs, recent research has utilized a variety of mechanical loading paradigms, including cyclic tensile strain, cyclic hydrostatic pressure, and mechanical unloading in simulated microgravity. This chapter will describe the methods for applying these mechanical stimuli to hASC to direct differentiation for functional tissue engineering of the musculoskeletal system.
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Acknowledgments
This work was funded by a North Carolina Space Grant Fellowship (R.N.), NSF/CBET Grant 1133427 (E.L.), William R. Kenan Jr. Institute for Engineering, Technology and Science grant (E.L.), NIH CTSA grant 550KR71418 (E.L.), and NIH CTSA grant 550KR61325 (E.L.). We also thank Alison Nordberg for drawing the figures.
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Nordberg, R.C., Bodle, J.C., Loboa, E.G. (2024). Mechanical Stimulation of Adipose-Derived Stromal/Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_25
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DOI: https://doi.org/10.1007/978-1-0716-3762-3_25
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