Abstract
Intravital microscopy allows a direct visualization of cells’ behavior in their environment in a living organism with all its complexity. With appropriated models, longitudinal studies of structural and functional changes can be followed in the same animal on long period. In the field of cancer, the dorsal window chamber model is the model of choice for tumor events such as cells migration, vessels growth, and their permeability or interactions between cells and vessels. Coupled with wide-field, confocal, or multiphoton fluorescence microscopes, high spatial and temporal resolutions of the cellular events can be analyzed in vivo.
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Acknowledgments
We thank Dr. Philippe Guy and Dr. Véronique LeCabec for the acquisition of the pictures of Fig. 3, Dr. Sophie Chabot for Fig. 4, Dr. Sandrine Pelofy for Fig. 5, and Dr. Bostjan Markelc for Fig. 6. We acknowledge the IPBS Genotoul core facilities, ANEXPLO and TRI, member of the national infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04).
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Bellard, E., Golzio, M. (2024). Cancer Imaging by Intravital Microscopy: The Dorsal Window Chamber Model. In: Čemažar, M., Jesenko, T., Lampreht Tratar, U. (eds) Mouse Models of Cancer. Methods in Molecular Biology, vol 2773. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3714-2_12
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DOI: https://doi.org/10.1007/978-1-0716-3714-2_12
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