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Oncogene-Driven Induction of Orthotopic Cholangiocarcinoma in Mice

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Liver Carcinogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2769))

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Abstract

Cholangiocarcinoma (CCA) is a malignancy affecting the epithelial cells that line the bile ducts. This cancer shows a poor prognosis and current treatments remain inefficient. Orthotopic CCA mouse models are useful for the development of innovative therapeutic strategies. Here, we describe an orthotopic model of intrahepatic CCA that can be easily induced in mice within 5 weeks at a high incidence. It is achieved by expressing two oncogenes, namely, (i) the intracellular domain of the Notch1 receptor (NICD) and (ii) AKT, in hepatocytes by means of the slee** beauty transposon system. These plasmid vectors are delivered by hydrodynamic injection into the tail vein. The present chapter also describes how to perform magnetic resonance imaging (MRI) of the livers to visualize intrahepatic CCA nodules.

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Acknowledgments

J.G.P. is supported by the SIRIC Cancer Research and Personalized Medicine (CARPEM); Multi-Organism Institute (ITMO) Aviesan Cancer (National Alliance for Life Sciences and Health), Institut National du Cancer (INCa), and Fondation pour la Recherche Médicale (FRM). M.C.M. is supported by the SIRIC Cancer Research and Personalized Medicine (CARPEM). G.K. is supported by the Ligue contre le Cancer (équipe labellisée); Agence National de la Recherche (ANR) – Projets blancs; AMMICa US23/CNRS UMS3655; Association pour la recherche sur le cancer (ARC); Cancéropôle Ile-de-France; European Research Council Advanced Investigator Grant “ICD-Cancer,” FRM; a donation by Elior; Equipex Onco-Pheno-Screen; European Joint Programme on Rare Diseases (EJPRD); European Research Council (ICD-Cancer), European Union Horizon 2020 Projects Oncobiome and Crimson; Fondation Carrefour; Institut National du Cancer (INCa); Institut Universitaire de France; LabEx Immuno-Oncology (ANR-18-IDEX-0001); a Cancer Research ASPIRE Award from the Mark Foundation; the RHU Immunolife; Seerave Foundation; SIRIC Stratified Oncology Cell DNA Repair and Tumor Immune Elimination (SOCRATE); and SIRIC Cancer Research and Personalized Medicine (CARPEM).

Author information

Authors and Affiliations

  1. Centre de Recherche des Cordeliers, Equipe labellisée par la Ligue contre le cancer, Inserm U1138, Université Paris Cité, Sorbonne Université, Institut Universitaire de France, Paris, France

    Céleste Plantureux, Juliette Paillet, Maria Pérez-Lanzón, Guido Kroemer, Maria Chiara Maiuri & Jonathan Pol

  2. Metabolomics and Cell Biology Platforms, UMS AMMICa, Gustave Roussy, Villejuif, France

    Céleste Plantureux, Juliette Paillet, Maria Pérez-Lanzón, Guido Kroemer, Maria Chiara Maiuri & Jonathan Pol

  3. Faculté de Médecine, Université Paris-Saclay, Kremlin-Bicêtre, France

    Céleste Plantureux

  4. Laboratory of Human Lymphohematopoieisis, Imagine Institute, INSERM UMR 1163, Université Paris Cité, Paris, France

    Juliette Paillet

  5. Smart Immune, Paris, France

    Juliette Paillet

  6. Plateforme Imageries du Vivant, Université de Paris, PARCC, INSERM, Paris, France

    Gwennhael Autret

  7. Institut du Cancer Paris CARPEM, Department of Biology, Hôpital Européen Georges Pompidou, AP-HP, Paris, France

    Guido Kroemer

  8. Department of Molecular Medicine and Medical Biotechnologies, University of Napoli Federico II, Naples, Italy

    Maria Chiara Maiuri

Authors
  1. Céleste Plantureux
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  2. Juliette Paillet
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  3. Gwennhael Autret
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  4. Maria Pérez-Lanzón
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  5. Guido Kroemer
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  6. Maria Chiara Maiuri
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  7. Jonathan Pol
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Editor information

Editors and Affiliations

  1. Centre de Recherche des Cordeliers, Paris, France

    Guido Kroemer

  2. Centre de Recherche des Cordeliers, Paris, France

    Jonathan Pol

  3. Centre de Recherche des Cordeliers, Paris, France

    Isabelle Martins

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Plantureux, C. et al. (2024). Oncogene-Driven Induction of Orthotopic Cholangiocarcinoma in Mice. In: Kroemer, G., Pol, J., Martins, I. (eds) Liver Carcinogenesis. Methods in Molecular Biology, vol 2769. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3694-7_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3694-7_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3693-0

  • Online ISBN: 978-1-0716-3694-7

  • eBook Packages: Springer Protocols

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