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Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients

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Single Cell Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2752))

Abstract

The role of circulating tumor cell (CTC) clusters in the metastatic dissemination process is gaining increased attention. Besides homotypic clusters, heterotypic clusters that contain tumor cells admixed with normal cells are frequently observed in patients with solid tumors. Current methods used for cluster detection and enumeration do not allow an accurate estimation of the relative fractions of tumor cells. Here we describe a method for estimating tumor fraction of clusters including isolation and collection of single clusters, assessment of copy number alterations of single clusters by low-pass whole genome sequencing, and bioinformatic analysis of sequencing data.

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Authors and Affiliations

  1. Biomarkers Unit, Fondazione IRCCS, Istituto Nazionale dei Tumori di Milano, Milan, Italy

    Carolina Reduzzi, Marta Vismara, Marco Silvestri, Rosita Motta & Vera Cappelletti

  2. Experimental Medicine and Therapy Research, University Regensburg, Regensburg, Germany

    Thomas Schamberger

  3. Cellular and Molecular Diagnostics Group, Division of Personalized Cancer Therapy, Fraunhofer Institute of Toxicology and Experimental Medicine ITEM-R, Regensburg, Germany

    Bernhard M. Polzer

  4. Division of Hematology/Oncology, Weill Cornell Medicine, New York, United States

    Carolina Reduzzi

Authors
  1. Carolina Reduzzi
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  2. Marta Vismara
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  3. Thomas Schamberger
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  4. Marco Silvestri
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  5. Rosita Motta
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  6. Bernhard M. Polzer
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  7. Vera Cappelletti
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Corresponding author

Correspondence to Vera Cappelletti .

Editor information

Editors and Affiliations

  1. Department of Urology, University Hospital Regensburg, Regensburg, Germany

    Miodrag Gužvić

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Reduzzi, C. et al. (2024). Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer Patients. In: Gužvić, M. (eds) Single Cell Analysis. Methods in Molecular Biology, vol 2752. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3621-3_7

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  • DOI: https://doi.org/10.1007/978-1-0716-3621-3_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3620-6

  • Online ISBN: 978-1-0716-3621-3

  • eBook Packages: Springer Protocols

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