Abstract
RNA double-strand hybridization is a key player in gene expression regulation. Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base pairs with complementary messenger RNA. Fluorescence-based single-molecule methods allow to study RNA-RNA interaction under physiological conditions. Here is described, how the dissociation constant of RNA double strands can be determined by applying fluorescence correlation spectroscopy.
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Werner, A. (2024). RNA Double-Helix Hybridization Measured by Fluorescence Correlation Spectroscopy. In: Arluison, V., Valverde, C. (eds) Bacterial Regulatory RNA. Methods in Molecular Biology, vol 2741. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3565-0_9
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DOI: https://doi.org/10.1007/978-1-0716-3565-0_9
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3564-3
Online ISBN: 978-1-0716-3565-0
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