Abstract
Plant vascular pathogens use different ways to reach the xylem vessels and cause devastating diseases in plants. Resistant and tolerant plants have evolved various defense mechanisms against vascular pathogens. Inducible physico-chemical structures, such as the formation of tyloses and wall reinforcements with phenolic polymers, are very effective barriers that confine the pathogen and prevent colonization. Here, we use a combination of classical histochemistry along with bright-field and fluorescence microscopy and two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy to visualize and characterize wall reinforcements containing phenolic wall polymers, namely, lignin, ferulates, and suberin, which occur in different xylem vasculature in response to pathogen attack.
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Acknowledgments
Research was funded through MCIN/AEI/ 10.13039/501100011033 and “ERDF A way of making Europe” with grants PID2019-108595RB-I00 (NSC) and PID2020-118968RB-I00 (JR) and with the fellowship PRE2020-092086 (ALJ-J). WZ is a recipient of the China Scholarship Council fellowship (CSC NO.201906990041). Research at CRAG is also funded through the “Severo Ochoa Programme for Centres of Excellence in R&D” (CEX2019-000902-S by MCIN/AEI/10.13039/501100011033) and through the CERCA Programme/Generalitat de Catalunya.
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Zhang, W., Jiménez-Jiménez, Á., Capellades, M., Rencoret, J., Kashyap, A., Coll, N.S. (2024). Determination of De Novo Suberin-Lignin Ferulate Deposition in Xylem Tissue Upon Vascular Pathogen Attack. In: Agusti, J. (eds) Xylem. Methods in Molecular Biology, vol 2722. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3477-6_9
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DOI: https://doi.org/10.1007/978-1-0716-3477-6_9
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