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Antibody Selection via Phage Display in Microtiter Plates

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Phage Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2702))

Abstract

The most common and robust in vitro technology to generate monoclonal human antibodies is phage display. This technology is a widely used and powerful key technology for recombinant antibody selection. Phage display-derived antibodies are used as research tools, in diagnostic assays, and by 2022, 14 phage display-derived therapeutic antibodies were approved. In this review, we describe a fast high-throughput antibody (scFv) selection procedure in 96-well microtiter plates. The given detailed protocol allows the antibody selection (“panning”), screening, and identification of monoclonal antibodies in less than 2 weeks. Furthermore, we describe an on-rate panning approach for the selection of monoclonal antibodies with fast on-rates.

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Acknowledgments

This protocol is an updated and revised version of [16, 28].

Author information

Authors and Affiliations

  1. Institut für Biochemie, Biotechnologie und Bioinformatik, Departments Biotechnology and Medical Biotechnology, Technische Universität Braunschweig, Braunschweig, Germany

    Stephan Steinke, Kristian Daniel Ralph Roth, Maximilian Ruschig, Nora Langreder, Saskia Polten, Kai-Thomas Schneider, Rico Ballmann, Giulio Russo, Maren Schubert, Federico Bertoglio & Michael Hust

  2. Abcalis GmbH, Braunschweig, Germany

    Kilian Johannes Karl Zilkens

  3. Choose Life Biotech SA, Bellinzona, Switzerland

    Federico Bertoglio

Authors
  1. Stephan Steinke
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  2. Kristian Daniel Ralph Roth
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  3. Maximilian Ruschig
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  4. Nora Langreder
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  5. Saskia Polten
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  6. Kai-Thomas Schneider
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  7. Rico Ballmann
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  8. Giulio Russo
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  9. Kilian Johannes Karl Zilkens
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  10. Maren Schubert
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  11. Federico Bertoglio
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  12. Michael Hust
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Corresponding author

Correspondence to Michael Hust .

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Editors and Affiliations

  1. Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  2. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, Malaysia

    Theam Soon Lim

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Steinke, S. et al. (2023). Antibody Selection via Phage Display in Microtiter Plates . In: Hust, M., Lim, T.S. (eds) Phage Display. Methods in Molecular Biology, vol 2702. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3381-6_12

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  • DOI: https://doi.org/10.1007/978-1-0716-3381-6_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3380-9

  • Online ISBN: 978-1-0716-3381-6

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