Abstract
Human forebrain cortical neurons are essential for fundamental functions like memory and consciousness. Generation of cortical neurons from human pluripotent stem cells provides a great source for creating models specific to cortical neuron diseases and for develo** therapeutics. This chapter describes a detailed and robust method for generating human mature cortical neurons from stem cells in 3D suspension culture.
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Acknowledgments
This study was supported in part by NIH-NINDS (NS096282, NS076352, NS086604), NICHD (HD106197, HD090256), the National Medical Research Council of Singapore (MOH-000212, MOH-000207), Ministry of Education of Singapore (MOE2018-T2-2-103), Aligning Science Across Parkinson’s (ASAP-000301), the Bleser Family Foundation, and the Busta Foundation.
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Yan, Y., Zhang, SC. (2023). Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture. In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3287-1_1
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DOI: https://doi.org/10.1007/978-1-0716-3287-1_1
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