Abstract
Pneumocystis jirovecii causes pneumonia in immunocompromised patients. A major challenge in drug susceptibility testing and in understanding host/pathogen interactions is that Pneumocystis spp. are not viable in vitro. Continuous culture of the organism is not currently available, and therefore, develo** new drug targets is very limited. Due to this limitation, mouse models of Pneumocystis pneumonia have proven to be an invaluable resource to researchers. In this chapter, we provide an overview of selected methods used in mouse models of infection including, in vivo Pneumocystis murina propagation, routes of transmission, genetic mouse models available, a P. murina life form-specific model, a mouse model of PCP immune reconstitution inflammatory syndrome (IRIS), and the experimental parameters associated with these models.
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Hoving, J.C., Munyonho, F.T., Kolls, J.K. (2023). Genetic Mouse Models of Pneumocystis Pneumonia. In: Drummond, R.A. (eds) Antifungal Immunity. Methods in Molecular Biology, vol 2667. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3199-7_13
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DOI: https://doi.org/10.1007/978-1-0716-3199-7_13
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