Abstract
“Immunoelectron microscopy” defines a group of techniques developed for visualizing where components of cells or tissues are localized, by means of a transmission electron microscope (TEM) at a subcellular resolution. The method is based on antigen recognition by primary antibodies and subsequent visualization of recognized structures by means of electron-opaque gold granules, which are easily visible in TEM images. The potentially high resolution of this method relies on the very small size of the colloidal gold label, which consists of granules ranging from 1 to 60 nm in diameter, mostly used in the 5–15 nm sizes.
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References
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Acknowledgments
The author is grateful to Dr. Nicoletta Zini (CNR Institute of Molecular Genetics “Luigi Luca Cavalli-Sforza,” Unit of Bologna) and Dr. Marta Columbaro (IRCCS Istituto Ortopedico Rizzoli, Bologna) for development of the technique and to Mrs. Patrizia Sabatelli and Mrs. Despina Kiriakidu (CNR Institute of Molecular Genetics “Luigi Luca Cavalli-Sforza,” Unit of Bologna) for technical assistance.
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Squarzoni, S. (2023). Immunoelectron Microscopy Methods. In: Lanzuolo, C., Marasca, F. (eds) Polycomb Group Proteins. Methods in Molecular Biology, vol 2655. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3143-0_15
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DOI: https://doi.org/10.1007/978-1-0716-3143-0_15
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