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CRISPR/LbCas12a-Mediated Genome Editing in Soybean

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Plant Genome Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2653))

Abstract

Currently methods for generating soybean edited lines are time-consuming, inefficient, and limited to certain genotypes. Here we describe a fast and highly efficient genome editing method based on CRISPR-Cas12a nuclease system in soybean. The method uses Agrobacterium-mediated transformation to deliver editing constructs and uses aadA or ALS genes as selectable marker. It only takes about 45 days to obtain greenhouse-ready edited plants at higher than 30% transformation efficiency and 50% editing rate. The method is applicable to other selectable markers including EPSPS and has low transgene chimera rate. The method is also genotype-flexible and has been applied to genome editing of several elite soybean varieties.

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Acknowledgments

The authors thank our transformation, plant analysis, and greenhouse colleagues Juan Wei, ** Jiang, Wenling Wang, Cheng** Zhang, Ling Zhu, Sabrina Patton, ** Xu, Lizhao Geng, Chunxia Liu, Huaibing ** & Yang Gao

  • Seeds Research, Syngenta Crop Protection, LLC, Research Triangle Park, NC, USA

    Heng Zhong, John Dawson, Bin Tian, Brenden Barco, **ujuan Su, Shujie Dong, Changbao Li, Sivamani Elumalai, Qiudeng Que, Ian Jepson & Liang Shi

    • Authors
    1. Dawei Liang
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    2. Yubo Liu
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    20. Liang Shi
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    Corresponding authors

    Correspondence to Dawei Liang or Qiudeng Que .

    Editor information

    Editors and Affiliations

    1. Division of Plant Sciences, University of Missouri, Columbia, MO, USA

      Bing Yang

    2. Department of Crop Genetics, John Innes Centre, Norwich, UK

      Wendy Harwood

    3. Syngenta Crop Protection, LLC., Research Triangle Park, NC, USA

      Qiudeng Que

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    © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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    Liang, D. et al. (2023). CRISPR/LbCas12a-Mediated Genome Editing in Soybean. In: Yang, B., Harwood, W., Que, Q. (eds) Plant Genome Engineering. Methods in Molecular Biology, vol 2653. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3131-7_3

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    • DOI: https://doi.org/10.1007/978-1-0716-3131-7_3

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    • Publisher Name: Humana, New York, NY

    • Print ISBN: 978-1-0716-3130-0

    • Online ISBN: 978-1-0716-3131-7

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