Abstract
The architectural structure of cells is essential for the cells’ function, which becomes especially apparent in the highly “structure functionally” tuned skeletal muscle cells. Here, structural changes in the microstructure can have a direct impact on performance parameters, such as isometric or tetanic force production. The microarchitecture of the actin-myosin lattice in muscle cells can be detected noninvasively in living cells and in 3D by using second harmonic generation (SHG) microscopy, forgoing the need to alter samples by introducing fluorescent probes into them. Here, we provide tools and step-by-step protocols to guide the processes of obtaining SHG microscopy image data from samples, as well as extracting characteristic values from the image data to quantify the cellular microarchitecture using characteristic patterns of myofibrillar lattice alignments.
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523551_2_En_17_MOESM3_ESM.stl
A stereolitography file for 3D reprinting of the single fiber chamber with perfusion capability “PrintableSingleFibreChamber.stl” is available under… (STL 481 kb)
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Schneidereit, D., Nübler, S., Friedrich, O. (2023). Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells. In: Friedrich, O., Gilbert, D.F. (eds) Cell Viability Assays. Methods in Molecular Biology, vol 2644. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3052-5_17
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DOI: https://doi.org/10.1007/978-1-0716-3052-5_17
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