Abstract
Inflammasomes are multiprotein complexes that can be activated by a wide array of infectious and inflammatory agents. Inflammasome activation culminates in the maturation and secretion of pro-inflammatory cytokines, as well as lytic cell death, known as pyroptosis. During pyroptosis, the entire contents of a cell are released into the extracellular space, propagating the local innate immune response. One component of particular interest is the alarmin high mobility group box-1 (HMGB1). Extracellular HMGB1 is a potent inflammatory stimulus, acting upon multiple receptors to drive inflammation. In this series of protocols, we will outline how to trigger and assess pyroptosis in primary macrophages, with a focus on the assessment of HMGB1 release.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Foley, A.E., Sulstarova, S., Goldenberg, N.M. (2023). Assessment of HMGB1 Release During Experimentally Induced Pyroptosis. In: Fink, S.L. (eds) Pyroptosis. Methods in Molecular Biology, vol 2641. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3040-2_16
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DOI: https://doi.org/10.1007/978-1-0716-3040-2_16
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3039-6
Online ISBN: 978-1-0716-3040-2
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