Abstract
Adult skeletal muscle is a dynamic tissue able to regenerate quite efficiently, thanks to the presence of stem cell machinery. Besides the quiescent satellite cells that are activated upon injury or paracrine factors, other stem cells are described to be directly or indirectly involved in adult myogenesis. Mesoangioblasts (MABs) are vessel-associated stem cells originally isolated from embryonic dorsal aorta and, at later stages, from the adult muscle interstitium expressing pericyte markers. Adult MABs entered clinical trials for the treatment of Duchenne muscular dystrophy and the transcriptome of human fetal MABs has been described. In addition, single cell RNA-seq analyses provide novel information on adult murine MABs and more in general in interstitial muscle stem cells. This chapter provides state-of-the-art techniques to isolate and characterize murine MABs, fetal and adult human MABs.
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Acknowledgments
Work in the authors’ laboratory is supported by The Research Foundation Flanders (FWO) (#G066821N), INTERREG – Euregio Meuse-Rhine (GYM, Generate your muscle 2020-EMR116), and Italian Ministry of Health, Ricerca Finalizzata (RF-2019-12369703). MS is recipient of Hercules Foundation grant (AKUL/19/34) for the financing provided to purchase the high throughput calcium imaging system.
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Giarratana, N., Conti, F., Rinvenuti, L., Ronzoni, F., Sampaolesi, M. (2023). State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts. In: Asakura, A. (eds) Skeletal Muscle Stem Cells. Methods in Molecular Biology, vol 2640. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3036-5_8
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DOI: https://doi.org/10.1007/978-1-0716-3036-5_8
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