Abstract
Skeletal muscle is a highly plastic tissue that can alter its mass and strength in response to mechanical stimulation, such as overloading and unloading, which lead to muscle hypertrophy and atrophy, respectively. Mechanical loading in the muscle influences muscle stem cell dynamics, including activation, proliferation, and differentiation. Although experimental models of mechanical overloading and unloading have been widely used for the investigation of the molecular mechanisms regulating muscle plasticity and stem cell function, few studies have described the methods in detail. Here, we describe the appropriate procedures for tenotomy-induced mechanical overloading and tail-suspension-induced mechanical unloading, which are the most common and simple methods to induce muscle hypertrophy and atrophy in mouse models.
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Acknowledgments
This work was supported by the Japan Agency for Medical Research and Development (AMED, JP18ek0109383 and JP19bm0704036), the FOREST program of the Japan Science and Technology Agency (JST, JPMJFR205C) the Grant-in-Aid for Scientific Research KAKENHI (18H03193, 20K21763, 20K19641, 22K18414 and 22H00505) from the Japan Society for the Promotion of Science (JSPS), the Uehara Memorial Foundation, and the Takeda Science Foundation.
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Fujimaki, S., Ono, Y. (2023). Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloading. In: Asakura, A. (eds) Skeletal Muscle Stem Cells. Methods in Molecular Biology, vol 2640. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3036-5_15
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DOI: https://doi.org/10.1007/978-1-0716-3036-5_15
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