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Quantitative Proteomics of Nervous System Regeneration: From Sample Preparation to Functional Data Analyses

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Axon Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2636))

Abstract

Mammals have a limited regenerative capacity, especially of the central nervous system. Consequently, any traumatic injury or neurodegenerative disease results in irreversible damage. An important approach to finding strategies to promote regeneration in mammals has been the study of regenerative organisms like Xenopus, the axolotl, and teleost fish. High-throughput technologies like RNA-Seq and quantitative proteomics are starting to provide valuable insight into the molecular mechanisms that drive nervous system regeneration in these organisms. In this chapter, we present a detailed protocol for performing iTRAQ proteomics that can be applied to the analysis of nervous system samples, using Xenopus laevis as an example. The quantitative proteomics protocol and directions for performing functional enrichment data analyses of gene lists (e.g., differentially abundant proteins from a proteomic study, or any type of high-throughput analysis) are aimed at the general bench biologist and do not require previous programming knowledge.

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Acknowledgments

This work was funded by FONDECYT Postdoctoral 3180180 (DLL). Figures 1 and 2 were created with BioRender.com.

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Authors and Affiliations

  1. Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile

    Dasfne Lee-Liu

  2. Department of Chemistry, Michigan State University, East Lansing, MI, USA

    Liangliang Sun

Authors
  1. Dasfne Lee-Liu
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  2. Liangliang Sun
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Corresponding author

Correspondence to Dasfne Lee-Liu .

Editor information

Editors and Affiliations

  1. Department of Biology, Appalachian State University, Boone, NC, USA

    Ava J. Udvadia

  2. Office of Technology Development, Medical College of Wisconsin, Milwaukee, WI, USA

    James B. Antczak

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Lee-Liu, D., Sun, L. (2023). Quantitative Proteomics of Nervous System Regeneration: From Sample Preparation to Functional Data Analyses. In: Udvadia, A.J., Antczak, J.B. (eds) Axon Regeneration. Methods in Molecular Biology, vol 2636. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3012-9_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3012-9_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3011-2

  • Online ISBN: 978-1-0716-3012-9

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