Abstract
Stable isotope probing (SIP) provides the opportunity to label decomposer microorganisms that build their biomass on a specific substrate. In combination with high-throughput sequencing, SIP allows for the identification of microbial community members involved in a particular decomposition process. Further information can be gained (in SIP experiments) through gene-targeted metagenomics and metatranscriptomics, opening the possibility to describe the pool of genes catalyzing specific decomposition reactions in situ and to identify the diversity of genes that are expressed. When combined with gene descriptions of fungal and/or bacterial isolates from the same environment, specific biochemical reactions involved in decomposition can be linked to individual microbial taxa. Here, we describe the use of these methods to explore the decomposer community of fungi and bacteria in forest litter and soil.
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This work was supported by the Czech Science Foundation (20-14961S and 22-30769S).
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Štursová, M., López-Mondéjar, R., Baldrian, P. (2023). Investigating the Bacterial and Fungal Communities Involved in Dead Biomass Degradation in Forest Soils. In: Martin, F., Uroz, S. (eds) Microbial Environmental Genomics (MEG). Methods in Molecular Biology, vol 2605. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2871-3_8
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DOI: https://doi.org/10.1007/978-1-0716-2871-3_8
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