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Methacrylated Gelatin as an On-Demand Injectable Vehicle for Drug Delivery in Dentistry

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2588))

Abstract

Gelatin methacrylate (GelMA) is a biodegradable and biocompatible engineered material with significant promise for its applications in tissue engineering, drug delivery, and 3D bioprinting applications. Gelatin is functionalized with terminal methacrylate groups which allow for its photoinducible crosslinking, and thereby tunable properties. Photocrosslinking of GelMA solution in situ allows for fabrication of hydrogels to fit patient-specific defects. Given its favorable biologic properties, GelMA may be used as a carrier for bioactive substances necessary to induce regenerative phenotypes or augment healing, such as growth factors and biotherapeutics. Gelatin is cleaved by cell-secreted enzymes such that its degradation, and subsequently release of bioactive substances, is well-matched to tissue regeneration processes. GelMA may be mixed with a wide array of additives to enhance and improve the specificity of its biologic activity. Here, we present two protocols for novel fabrications and their uses as clinically relevant drug delivery systems. GelMA hydrogels provides a versatile platform for the development of injectable drug delivery therapeutics for broad applications in regenerative dental medicine.

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Acknowledgments

M.C.B. acknowledges the National Institutes of Health (NIH – National Institute of Dental and Craniofacial Research/NIDCR, R01DE026578). M.C.B. is grateful for funds received from the OsteoScience Foundation (Peter Geistlich Research Award) and the American Academy of Implant Dentistry Foundation (AAIDF). W.B.S. acknowledges the NIH/NIDCR (F30DE029359) and American Academy of Implant Dentistry Foundation Student Research Fellowship (AAIDF). The authors are indebted to Luiz E. Bertassoni (Oregon Health & Science University, School of Dentistry) for sharing the protocol involved in the synthesis of methacrylated gelatin hydrogels. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and NSF. We apologize to colleagues whose work we could not discuss due to the space limitations.

Author information

Authors and Affiliations

  1. Department of Biologic and Materials Science and Division of Prosthodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA

    W. Benton Swanson & Seth Woodbury

  2. Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USA

    Abdel Hameed Mahmoud & Marco C. Bottino

  3. Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, MI, USA

    Marco C. Bottino

Authors
  1. W. Benton Swanson
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  2. Abdel Hameed Mahmoud
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  3. Seth Woodbury
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  4. Marco C. Bottino
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Corresponding author

Correspondence to Marco C. Bottino .

Editor information

Editors and Affiliations

  1. School of Dentistry, The University of Queensland, Herston, QLD, Australia

    Gregory J. Seymour

  2. School of Dentistry, The University of Queensland, Herston, QLD, Australia

    Mary P. Cullinan

  3. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

    Nicholas C.K. Heng

  4. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

    Paul R. Cooper

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Swanson, W.B., Mahmoud, A.H., Woodbury, S., Bottino, M.C. (2023). Methacrylated Gelatin as an On-Demand Injectable Vehicle for Drug Delivery in Dentistry. In: Seymour, G.J., Cullinan, M.P., Heng, N.C., Cooper, P.R. (eds) Oral Biology. Methods in Molecular Biology, vol 2588. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2780-8_30

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  • DOI: https://doi.org/10.1007/978-1-0716-2780-8_30

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2779-2

  • Online ISBN: 978-1-0716-2780-8

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