Abstract
T cells develop in the thymus from bone marrow precursors, and genetic manipulation is an indispensable tool to explore their development in vivo. Retroviral transduction of T cell precursors in the bone marrow can be used to specifically eliminate or enforce gene expression. Here, we describe a fast and efficient method to ectopically express a gene in T cell precursors through retroviral transduction and transplant into recipient mice, which will enable laboratories to evaluate gene function in T cell development in vivo.
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Gao, Y., Bosselut, R. (2023). Generation of Retrogenic Mice to Investigate T Cell Development. In: Bosselut, R., Vacchio, M.S. (eds) T-Cell Development. Methods in Molecular Biology, vol 2580. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2740-2_12
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DOI: https://doi.org/10.1007/978-1-0716-2740-2_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2739-6
Online ISBN: 978-1-0716-2740-2
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