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Analysis of Cell Cycle by Flow Cytometry

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Cell-Cycle Synchronization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2579))

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Abstract

The cell cycle of a cell is tightly controlled by several regulators. Dysregulation of cell cycle can lead to uncontrolled cell division which is one of the main characteristics of cancer cells. DNA content of a cell is changed during the cell cycle progression and can be measured by flow cytometry. In this chapter, we aim to provide a detailed protocol on how to analyze the cell cycle using flow cytometry.

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References

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Funding

This research has been funded by the generous support of the Stollery Children’s Hospital Foundation through the Women and Children’s Health Research Institute.

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Authors and Affiliations

  1. Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, AB, Canada

    Hamid Maadi

  2. Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran

    Mohammad Hasan Soheilifar

  3. Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada

    Zhixiang Wang

Authors
  1. Hamid Maadi
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  2. Mohammad Hasan Soheilifar
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  3. Zhixiang Wang
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Editor information

Editors and Affiliations

  1. Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada

    Zhixiang Wang

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cite this protocol

Maadi, H., Soheilifar, M.H., Wang, Z. (2022). Analysis of Cell Cycle by Flow Cytometry. In: Wang, Z. (eds) Cell-Cycle Synchronization. Methods in Molecular Biology, vol 2579. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2736-5_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2736-5_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2735-8

  • Online ISBN: 978-1-0716-2736-5

  • eBook Packages: Springer Protocols

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