Abstract
N-acylethanolamine-hydrolyzing acid amidase (NAAA) is a lysosomal hydrolase degrading various N-acylethanolamines at acidic pH. NAAA prefers anti-inflammatory and analgesic palmitoylethanolamide to other N-acylethanolamines as a substrate, and its specific inhibitors are shown to exert anti-inflammatory and analgesic actions in animal models. Therefore, these inhibitors are expected as a new class of therapeutic agents. Here, we introduce an NAAA assay system, using [14C]palmitoylethanolamide and thin-layer chromatography. The preparation of NAAA enzyme from native and recombinant sources as well as the chemical synthesis of N-[1′-14C]palmitoyl-ethanolamine is also described.
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Acknowledgments
We thank Iffat Ara Sonia Rahman, Zahir Hussain, and Smriti Sultana Binte Mustafiz for their careful reading of the manuscript and valuable suggestions.
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Tsuboi, K., Ueda, N. (2023). Assay of NAAA Activity. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 2576. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2728-0_22
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DOI: https://doi.org/10.1007/978-1-0716-2728-0_22
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