Abstract
Aptamers are single-stranded oligonucleotides able to recognize a target with high affinity and specificity. Aptamers are used in different diagnostics applications, highlighting, among all, variations of the traditional enzyme-linked immunosorbent assay (ELISA). In this chapter, we show the procedures for the development of two types of indirect ELONA: a sandwich ELONA and a direct ELONA coupled to either real-time quantitative PCR as a direct and sensitive readout.
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Moreno, M., García-Sacristán, A., Martín, M.E., González, V.M. (2023). Enzyme-Linked Oligonucleotide Assay (ELONA). In: Mayer, G., Menger, M.M. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 2570. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2695-5_18
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DOI: https://doi.org/10.1007/978-1-0716-2695-5_18
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