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Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues

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Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2566))

Abstract

Autophagy is crucial for maintaining cellular homeostasis and its deregulation is involved in disease development, including cancer. The key players of chaperone-mediated autophagy (CMA), a particular selective subtype of autophagy, are HSPA8 and LAMP2A. Both proteins can be immunohistochemically detected in formalin-fixed paraffin-embedded (FFPE) tissue. LAMP2A is frequently overexpressed in a variety of cancers where it likely supports cancer cell survival and resistance to anti-cancer therapies in a context-dependent manner. Here we present the immunohistochemical staining protocol of antibodies against LAMP2A and HSPA8, using an automated staining system, suitable for routine diagnostics. Additionally, we also suggest a staining evaluation method.

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Acknowledgments

We thank the Translational Research Unit (TRU) of the Institute of Pathology, University of Bern, for expert technical support.

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Authors and Affiliations

  1. Institute of Pathology, University of Bern, Bern, Switzerland

    Tereza Losmanová, Mario P. Tschan, José A. Galván & Sabina Berezowska

  2. Department of Laboratory Medicine and Pathology, Institute of Pathology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland

    Sabina Berezowska

Authors
  1. Tereza Losmanová
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  2. Mario P. Tschan
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  3. José A. Galván
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  4. Sabina Berezowska
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Corresponding author

Correspondence to Sabina Berezowska .

Editor information

Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, PAVIA, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, PAVIA, Italy

    Marco Biggiogera

  3. Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, VERONA, Italy

    Manuela Malatesta

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Losmanová, T., Tschan, M.P., Galván, J.A., Berezowska, S. (2023). Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues. In: Pellicciari, C., Biggiogera, M., Malatesta, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 2566. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2675-7_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2675-7_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2674-0

  • Online ISBN: 978-1-0716-2675-7

  • eBook Packages: Springer Protocols

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