Abstract
Fundus autofluorescence (FAF) imaging is a noninvasive retinal imaging methodology that allows map** of lipofuscin distribution in the retinal pigment epithelium cell (RPE). Excessive accumulation of lipofuscin granules in the lysosomal compartment of RPE cells represents a common downstream pathogenetic pathway in various hereditary and complex retinal diseases, including age-related macular degeneration. The clinical applications of FAF coupled with its ease of use, and the noninvasive nature of characterizing retinal diseases, are increasingly valuable to the field of ophthalmology and in assessing the progression of retinitis pigmentosa (RP). Quantitative AF (qAF) enhances the understanding of retinal disease processes, serves as a diagnostic aid, and allows for the monitoring of the effects of therapeutic interventions. This chapter introduces basic principles of FAF and general protocols of FAF evaluating retinal disease progression in rodents.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Pichi F, Abboud EB, Ghazi NG et al (2018) Fundus autofluorescence imaging in hereditary retinal diseases. Acta Ophthalmol 96:e549–e561. https://doi.org/10.1111/aos.13602
Sparrow JR, Wu Y, Nagasaki T et al (2010) Fundus autofluorescence and the bisretinoids of retina. Photochem Photobiol Sci 9:1480–1489. https://doi.org/10.1039/c0pp00207k
Schmitz-Valckenberg S, Holz FG, Bird AC et al (2008) Fundus autofluorescence imaging: review and perspectives. Retina 28:385–409. https://doi.org/10.1097/IAE.0b013e318164a907
Zhang L, Cui X, Jauregui R et al (2018) Genetic rescue reverses microglial activation in preclinical models of retinitis pigmentosa. Mol Ther 26:1953–1964. https://doi.org/10.1016/j.ymthe.2018.06.014
Delori F, Greenberg JP, Woods RL et al (2011) Quantitative measurements of autofluorescence with the scanning laser ophthalmoscope. Invest Ophthalmol Vis Sci 52:9379–9390. https://doi.org/10.1167/iovs.11-8319
Sparrow JR, Blonska A, Flynn E et al (2013) Quantitative fundus autofluorescence in mice: correlation with HPLC quantitation of RPE lipofuscin and measurement of retina outer nuclear layer thickness. Invest Ophthalmol Vis Sci 54:2812–2820. https://doi.org/10.1167/iovs.12-11490
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Ethics declarations
Stephen H. Tsang receives financial support from Abeona Therapeutics, Inc and Emendo. He is also the founder of Rejuvitas and is on the scientific and clinical advisory board for Nanoscope Therapeutics.
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Cheng, CH., Cui, X., Tsang, S.H. (2023). Autofluorescence Imaging to Evaluate Retinal Disease Progression in Rodent. In: Tsang, S.H., Quinn, P.M. (eds) Retinitis Pigmentosa. Methods in Molecular Biology, vol 2560. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2651-1_21
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2651-1_21
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2650-4
Online ISBN: 978-1-0716-2651-1
eBook Packages: Springer Protocols