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Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer

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Chromosome Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2519))

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Abstract

Hi-C is a method that analyzes genome-wide chromatin structure using next-generation sequencer. Chromatin structure is crucial for regulating transcription or replication, and Hi-C has revealed the hierarchical chromatin structures, such as loop, domain , and compartment structures. Aberrant alteration of these structures causes disease, and a number of structural aberrations in cancer cells have been reported recently. Besides, Hi-C can identify chromosome rearrangements that frequently occurred in cancer. Therefore, Hi-C is a powerful technique to analyze epigenomic and genomic aberrations in tumorigenesis. Here we will introduce the basic protocol of Hi-C in experimental and analytical aspects.

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Authors and Affiliations

  1. Department of Molecular Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan

    Atsushi Okabe & Atsushi Kaneda

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  1. Atsushi Okabe
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  2. Atsushi Kaneda
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Correspondence to Atsushi Kaneda .

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Editors and Affiliations

  1. Division of Diagnostic Imaging, Department of Radiology, Japan Labour Health and Safety Organization, Tokyo Rosai Hospital, Ohta-ku, Tokyo, Japan

    Eisuke Gotoh

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Okabe, A., Kaneda, A. (2023). Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer. In: Gotoh, E. (eds) Chromosome Analysis. Methods in Molecular Biology, vol 2519. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2433-3_15

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  • DOI: https://doi.org/10.1007/978-1-0716-2433-3_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2432-6

  • Online ISBN: 978-1-0716-2433-3

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