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Defined Engineered Human Myocardium for Disease Modeling, Drug Screening, and Heart Repair

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Cardiac Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2485))

Abstract

Different engineered heart muscle formats have been developed for applications in disease modeling, drug screening, and heart repair. The advantage of 3D engineered versus 2D monolayer and 3D aggregate cardiomyocyte cultures is a clearly advanced degree of maturation, which in many aspects resembles the postnatal rather than the embryonic or fetal heart, in the most advanced 3D culture formats. According to the desired in vitro (disease modeling or drug screening) and in vivo (heart repair) application, scale and geometry of tissue engineered heart muscle must be adapted. In this updated methods paper, we report a simple and scalable (up and down) collagen-based protocol for the construction of Engineered Human Myocardium (EHM) under defined, serum-free conditions.

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Acknowledgments

M.T. is supported by the German Research Foundation (DFG TI 956/1-1, SFB 1002 C04). T.M. is supported by the DZHK (German Center for Cardiovascular Research; Shared Expertise Funding Scheme). P.L.S. is a member of DFG IRTG 1816. W.H.Z. is supported by the DZHK, the Leducq Foundation, the German Ministry for Science and Education (BMBF - IndiHeart), and the German Research Foundation (DFG SFB 1002 C04 and S01, IRTG 1816, MBExC - EXC 2067/1).

Author information

Authors and Affiliations

  1. Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany

    Malte Tiburcy, Tim Meyer, Pierre-Luc Satin & Wolfram-Hubertus Zimmermann

  2. DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, Göttingen, Germany

    Malte Tiburcy, Tim Meyer, Pierre-Luc Satin & Wolfram-Hubertus Zimmermann

  3. Cluster of Excellence “Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells” (MBExC), University of Göttingen, Göttingen, Germany

    Wolfram-Hubertus Zimmermann

  4. Center for Neurodegenerative Diseases (DZNE), Göttingen, Germany

    Wolfram-Hubertus Zimmermann

  5. Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Göttingen, Germany

    Wolfram-Hubertus Zimmermann

Authors
  1. Malte Tiburcy
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  2. Tim Meyer
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  3. Pierre-Luc Satin
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  4. Wolfram-Hubertus Zimmermann
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Corresponding author

Correspondence to Wolfram-Hubertus Zimmermann .

Editor information

Editors and Affiliations

  1. School of Engineering, Brown University, Providence, RI, USA

    Kareen L.K. Coulombe

  2. Department of Biomedical Engineering, Tufts University, Medford, MA, USA

    Lauren D. Black III

Ethics declarations

M.T., T.M., and W.H.Z. are listed as inventors of several granted and pending patent applications in the field of stem cell differentiation, tissue engineering, and tool development. The University Medical Center Göttingen has licensed related IP to myriamed GmbH and Repairon GmbH. W.H.Z. is founder and advisor of myriamed GmbH (active in drug screening) and Repairon GmbH (active in the clinical translation of tissue engineered heart repair). M.T. and T.M. are advisors of myriamed GmbH and Repairon GmbH.

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Tiburcy, M., Meyer, T., Satin, PL., Zimmermann, WH. (2022). Defined Engineered Human Myocardium for Disease Modeling, Drug Screening, and Heart Repair. In: Coulombe, K.L., Black III, L.D. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 2485. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2261-2_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2261-2_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2260-5

  • Online ISBN: 978-1-0716-2261-2

  • eBook Packages: Springer Protocols

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