Abstract
The ncRNAs are short RNA transcripts with sequence and structure resembling that of specific domains in the non-coding regions of the foot-and-mouth disease (FMD) virus (FMDV ) genome. These synthetic molecules induce a robust antiviral response and have been shown to enhance the immune response and protection induced by an FMD inactivated vaccine in pigs. Here, we describe the method for ncRNAs synthesis, formulation, and delivery into mice and pigs for studies focused on testing the adjuvant effect of RNA-based strategies in combination with veterinarian vaccines.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
OIE (2020). https://www.oie.int/en/animal-health-in-the-world/animal-diseases/Foot-and-mouth-disease/
Knight-Jones TJ, Robinson L, Charleston B et al (2016) Global foot-and-mouth disease research update and gap analysis: 2—epidemiology, wildlife and economics. Transbound Emerg Dis 63 Suppl 1: 14–29
Saiz M, Nunez JI, Jimenez-Clavero MA et al (2002) Foot-and-mouth disease virus: biology and prospects for disease control. Microbes Infect 4:1183–1192
Nfon C, Lung O, Embury-Hyatt C et al (2017) Clinical signs and pathology of foot-and-mouth disease virus. In: Sobrino F, Domingo E (eds) Foot-and-mouth disease virus. Current research and emerging trends. Caister Academic Press, Norfolk, pp 171–178
Martinez-Salas E, Francisco-Velilla R, Fernandez-Chamorro J et al (2015) Picornavirus IRES elements: RNA structure and host protein interactions. Virus Res 206:62–73
Rodriguez-Pulido M, Sobrino F, Borrego B et al (2009) Attenuated foot-and-mouth disease virus RNA carrying a deletion in the 3′ noncoding region can elicit immunity in swine. J Virol 83:3475–3485
Borrego B, Rodriguez-Pulido M, Revilla C et al (2015) Synthetic RNAs mimicking structural domains in the foot-and-mouth disease virus genome elicit a broad innate immune response in porcine cells triggered by RIG-I and TLR activation. Viruses 7:3954–3973
Lorenzo G, Rodriguez-Pulido M, Lopez-Gil E et al (2014) Protection against Rift Valley fever virus infection in mice upon administration of interferon-inducing RNA transcripts from the FMDV genome. Antivir Res 109:64–67
Rodriguez-Pulido M, Martin-Acebes MA, Escribano-Romero E et al (2012) Protection against West Nile virus infection in mice after inoculation with type I interferon-inducing RNA transcripts. PLoS One 7:e49494
Rodriguez-Pulido M, Sobrino F, Borrego B et al (2011) Inoculation of newborn mice with non-coding regions of foot-and-mouth disease virus RNA can induce a rapid, solid and wide-range protection against viral infection. Antivir Res 92:500–504
Rodriguez-Pulido M, Ranjitha HB, Saiz M (2020) A wide-ranging antiviral response in wild boar cells is triggered by non-coding synthetic RNAs derived from the foot-and-mouth disease virus genome. Front Vet Sci 7:496
Borrego B, Rodriguez-Pulido M, Mateos F et al (2013) Delivery of synthetic RNA can enhance the immunogenicity of vaccines against foot-and-mouth disease virus (FMDV) in mice. Vaccine 31:4375–4381
Borrego B, Blanco E, Rodriguez-Pulido M et al (2017) Combined administration of synthetic RNA and a conventional vaccine improves immune responses and protection against foot-and-mouth disease virus in swine. Antivir Res 142:30–36
Rodriguez-Pulido M, Borrego B, Sobrino F et al (2011) RNA structural domains in non-coding regions of foot-and-mouth disease virus genome trigger innate immunity in porcine cells and mice. J Virol 85:6492–6501
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Rodríguez-Pulido, M., Polo, M., Borrego, B., Sáiz, M. (2022). Use of Foot-and-Mouth Disease Virus Non-coding Synthetic RNAs as Vaccine Adjuvants. In: Brun, A. (eds) Vaccine Technologies for Veterinary Viral Diseases. Methods in Molecular Biology, vol 2465. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2168-4_7
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2168-4_7
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2167-7
Online ISBN: 978-1-0716-2168-4
eBook Packages: Springer Protocols